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Single-particle imaging without symmetry constraints at an X-ray free-electron laser
DESY, Notkestr 85, D-22607 Hamburg, Germany..
Natl Res Ctr, Kurchatov Inst, Akad Kurchatova Pl 1, Moscow 123182, Russia..
Ctr Free Electron Laser Sci CFEL, Notkestr 85, D-22607 Hamburg, Germany..
European XFEL GmbH, Holzkoppel 4, D-22869 Schenefeld, Germany..
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2018 (English)In: IUCrJ, ISSN 0972-6918, E-ISSN 2052-2525, Vol. 5, p. 727-736Article in journal (Refereed) Published
Abstract [en]

The analysis of a single-particle imaging (SPI) experiment performed at the AMO beamline at LCLS as part of the SPI initiative is presented here. A workflow for the three-dimensional virus reconstruction of the PR772 bacteriophage from measured single-particle data is developed. It consists of several well defined steps including single-hit diffraction data classification, refined filtering of the classified data, reconstruction of three-dimensional scattered intensity from the experimental diffraction patterns by orientation determination and a final three-dimensional reconstruction of the virus electron density without symmetry constraints. The analysis developed here revealed and quantified nanoscale features of the PR772 virus measured in this experiment, with the obtained resolution better than 10 nm, with a clear indication that the structure was compressed in one direction and, as such, deviates from ideal icosahedral symmetry.

Place, publisher, year, edition, pages
INT UNION CRYSTALLOGRAPHY , 2018. Vol. 5, p. 727-736
Keywords [en]
single-particle imaging, three-dimensional virus reconstructions, XFELs
National Category
Physical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-239079DOI: 10.1107/S205225251801120XISI: 000448982300010Scopus ID: 2-s2.0-85056165262OAI: oai:DiVA.org:kth-239079DiVA, id: diva2:1264915
Funder
Swedish Research CouncilSwedish Foundation for Strategic Research
Note

QC 20181121

Available from: 2018-11-21 Created: 2018-11-21 Last updated: 2018-11-21Bibliographically approved

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Sellberg, Jonas A.

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