Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A systems-approach reveals human nestin is an endothelial-enriched, angiogenesis-independent intermediate filament protein
KTH, Centres, Science for Life Laboratory, SciLifeLab. KTH, School of Biotechnology (BIO).
KTH, Centres, Science for Life Laboratory, SciLifeLab. KTH, School of Biotechnology (BIO).ORCID iD: 0000-0003-0198-7137
Show others and affiliations
2018 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 8, no 1, article id 14668Article in journal (Refereed) Published
Abstract [en]

The intermediate filament protein nestin is expressed during embryonic development, but considered largely restricted to areas of regeneration in the adult. Here, we perform a body-wide transcriptome and protein-profiling analysis to reveal that nestin is constitutively, and highly-selectively, expressed in adult human endothelial cells (EC), independent of proliferative status. Correspondingly, we demonstrate that it is not a marker for tumour EC in multiple malignancy types. Imaging of EC from different vascular beds reveals nestin subcellular distribution is shear-modulated. siRNA inhibition of nestin increases EC proliferation, and nestin expression is reduced in atherosclerotic plaque neovessels. eQTL analysis reveals an association between SNPs linked to cardiovascular disease and reduced aortic EC nestin mRNA expression. Our study challenges the dogma that nestin is a marker of proliferation, and provides insight into its regulation and function in EC. Furthermore, our systems-based approach can be applied to investigate body-wide expression profiles of any candidate protein. 

Place, publisher, year, edition, pages
Nature Publishing Group , 2018. Vol. 8, no 1, article id 14668
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-236564DOI: 10.1038/s41598-018-32859-4ISI: 000446034000069PubMedID: 30279450Scopus ID: 2-s2.0-85054173189OAI: oai:DiVA.org:kth-236564DiVA, id: diva2:1266058
Note

Export Date: 22 October 2018; Article; Correspondence Address: Butler, L.M.; Science for Life Laboratory, School of Biotechnology, Kungliga Tekniska Högskolan (KTH) Royal Institute of TechnologySweden; email: Lynn.Butler@ki.se. QC 20181127

Available from: 2018-11-27 Created: 2018-11-27 Last updated: 2020-03-09Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Fagerberg, LinnUhlén, MathiasOdeberg, Jacob

Search in DiVA

By author/editor
Dusart, PhilipFagerberg, LinnStruck, EikeUhlén, MathiasOdeberg, JacobButler, Lynn M.
By organisation
Science for Life Laboratory, SciLifeLabSchool of Biotechnology (BIO)
In the same journal
Scientific Reports
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 28 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf