Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Forces drive basement membrane invasion in Caenorhabditis elegans
PSL Res Univ, CNRS, Lab Physicochim Curie, Inst Curie, F-75005 Paris, France.;Univ Paris 05, F-75005 Paris, France.;Sorbonne Univ, F-75005 Paris, France..
PSL Res Univ, CNRS, Lab Physicochim Curie, Inst Curie, F-75005 Paris, France.;Sorbonne Univ, F-75005 Paris, France..
Duke Univ, Dept Biol, Regenerat Next, Durham, NC 27705 USA..
KTH, School of Engineering Sciences (SCI), Applied Physics. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0002-9596-5125
Show others and affiliations
2018 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 115, no 45, p. 11537-11542Article in journal (Refereed) Published
Abstract [en]

During invasion, cells breach basement membrane (BM) barriers with actin-rich protrusions. It remains unclear, however, whether actin polymerization applies pushing forces to help break through BM, or whether actin filaments play a passive role as scaffolding for targeting invasive machinery. Here, using the developmental event of anchor cell (AC) invasion in Caenorhabditis elegans, we observe that the AC deforms the BM and underlying tissue just before invasion, exerting forces in the tens of nanonewtons range. Deformation is driven by actin polymerization nucleated by the Arp2/3 complex and its activators, whereas formins and cross-inkers are dispensable. Delays in invasion upon actin regulator loss are not caused by defects in AC polarity, trafficking, or secretion, as appropriate markers are correctly localized in the AC even when actin is reduced and invasion is disrupted. Overall force production emerges from this study as one of the main tools that invading cells use to promote BM disruption in C. elegans.

Place, publisher, year, edition, pages
National Academy of Sciences , 2018. Vol. 115, no 45, p. 11537-11542
Keywords [en]
actin cytoskeleton, force production, cell invasion, Arp2/3 complex, anchor cell
National Category
Other Engineering and Technologies
Identifiers
URN: urn:nbn:se:kth:diva-239479DOI: 10.1073/pnas.1808760115ISI: 000449459000066PubMedID: 30348801Scopus ID: 2-s2.0-85056145489OAI: oai:DiVA.org:kth-239479DiVA, id: diva2:1266182
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience, MoNaLISA 638314EU, European Research Council
Note

QC 20181127

Available from: 2018-11-27 Created: 2018-11-27 Last updated: 2019-03-18Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Dreier, JesTesta, Ilaria

Search in DiVA

By author/editor
Dreier, JesTesta, Ilaria
By organisation
Applied PhysicsScience for Life Laboratory, SciLifeLab
In the same journal
Proceedings of the National Academy of Sciences of the United States of America
Other Engineering and Technologies

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 33 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf