A computational systems biology study of the lambda-lac mutants
2007 (English)Manuscript (preprint) (Other academic)
We present a comprehensive computational study of some 900 possible “λ-lac” mutants of thelysogeny maintenance switch in phage λ, of which up to date 19 have been studied experimentally(Atsumi & Little, PNAS 103: 4558-4563, (2006)). We clarify that these mutants realise regulatoryschemes quite different from wild-type λ, and can therefore be expected to behave differently, withinthe conventional mechanistic setting in which this problem has often been framed. We verify thatindeed, within this framework, across this wide selection of mutants the λ-lac mutants for the mostpart either have no stable lytic states, or should only be inducible with difficulty. In particular, thecomputational results contradicts the experimental finding that four λ-lac mutants both show stablelysogeny and are inducible. This work hence suggests either that the four out of 900 mutants are special,or that λ lysogeny and inducibility are holistic effects involving other molecular players or othermechanisms, or both. The approach illustrates the power and versatility of computational systemsbiology to systematically and quickly test a wide variety of examples and alternative hypotheses for future closer experimental studies.
Place, publisher, year, edition, pages
Condensed Matter Physics
IdentifiersURN: urn:nbn:se:kth:diva-7605OAI: oai:DiVA.org:kth-7605DiVA: diva2:12685
QC 201011192007-11-122007-11-122016-02-02Bibliographically approved