Segregation to non-dividing cells in recombinant Escherichia coli fed-batch fermentation processes
2004 (English)In: Biotechnology letters, ISSN 0141-5492, E-ISSN 1573-6776, Vol. 26, no 19, 1533-1539 p.Article in journal (Refereed) Published
In Escherichia coli fermentation processes, a drastic drop in viable cell count as measured by the number of colony forming units per ml (c.f.u. ml(-1)) is often observed. This phenomenon was investigated in a process for the production of the recombinant fusion protein, promegapoietin (PMP). After induction, the number of c.f.u. ml(-1) dropped to similar to10% of its maximum though the biomass concentration continued to increase. Flow cytometric analysis of viability and intracellular concentration of PMP showed that almost all cells were alive and contributed to the production. Thus, the drop in the number of c.f.u. ml(-1) probably reflects a loss of cell division capability rather than cell death.
Place, publisher, year, edition, pages
2004. Vol. 26, no 19, 1533-1539 p.
Bacterial cell division; Escherichia coli; Fed-batch fermentation; Flow cytometry; Viability; Biochemistry; Biomass; Cells; Cytology; Escherichia coli; Fermentation; Proteins; Cell culture; Bacteria (microorganisms); Escherichia coli; hybrid protein; interleukin 3; interleukin 3 receptor; promegapoietin 1a; promegapoietin-1a; thrombopoietin; apoptosis; article; bacterial count; bioreactor; biosynthesis; cell aggregation; cell survival; comparative study; cytology; Escherichia coli; evaluation; fermentation; flow cytometry; genetics; methodology; microbiology; mitosis; physiology; protein engineering; Apoptosis; Bioreactors; Cell Aggregation; Cell Survival; Colony Count, Microbial; Escherichia coli; Fermentation; Flow Cytometry; Interleukin-3; Mitosis; Protein Engineering; Receptors, Interleukin-3; Recombinant Fusion Proteins; Thrombopoietin
Other Industrial Biotechnology
IdentifiersURN: urn:nbn:se:kth:diva-7620DOI: 10.1023/B:BILE.0000044458.29147.75ISI: 000225490200014ScopusID: 2-s2.0-21644447627OAI: oai:DiVA.org:kth-7620DiVA: diva2:12704
QC 20100819 QC 201109222007-11-122007-11-122011-09-22Bibliographically approved