Monitoring and quantification of inclusion body formation in Escherichia coli by multi-parameter flow cytometry
2005 (English)In: Biotechnology letters, ISSN 0141-5492, E-ISSN 1573-6776, Vol. 27, no 13, 919-926 p.Article in journal (Refereed) Published
Multi-parameter flow cytometry was used to monitor the formation of promegapoietin (PMP) inclusion bodies during a high cell density Escherichia coli fed-batch fermentation process. Inclusion bodies were labelled with a primary antibody and then with a secondary fluorescent antibody. Using this method it was possible to detect PMP inclusion body formation with a high specificity and it was possible to monitor the increased accumulation of the protein with process time (6-48 mg PMP/g CDW) whilst highlighting population heterogeneity.
Place, publisher, year, edition, pages
2005. Vol. 27, no 13, 919-926 p.
Alexa fluor 488; Escherichia coli; Fed-batch fermentation; Flow cytometry; Inclusion bodies; Escherichia coli; Fermentation; Fluorescence; Cell density; Cytometry; Fluorescent antibodies; Inclusion bodies; Proteins; fluorescent dye; hybrid protein; interleukin 3; interleukin 3 receptor; promegapoietin 1a; promegapoietin-1a; recombinant protein; thrombopoietin; article; bioreactor; cell inclusion; chemistry; Escherichia coli; fermentation; flow cytometry; fluorescence microscopy; genetics; growth, development and aging; high performance liquid chromatography; human; metabolism; methodology; microbiology; Bioreactors; Chromatography, High Pressure Liquid; Escherichia coli; Fermentation; Flow Cytometry; Fluorescent Dyes; Humans; Inclusion Bodies; Interleukin-3; Microscopy, Fluorescence; Receptors, Interleukin-3; Recombinant Fusion Proteins; Recombinant Proteins; Thrombopoietin
Other Industrial Biotechnology
IdentifiersURN: urn:nbn:se:kth:diva-7621DOI: 10.1007/s10529-005-7184-6ISI: 000231164000007PubMedID: 16091887ScopusID: 2-s2.0-23844548756OAI: oai:DiVA.org:kth-7621DiVA: diva2:12705
QC 201008192007-11-122007-11-122010-08-19Bibliographically approved