Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A fluorescence nanoscopy marker for corticotropin-releasing hormone type 1 receptor: computer design, synthesis, signaling effects, super-resolved fluorescence imaging, and in situ affinity constant in cells
Consejo Nacl Invest Cient & Tecn, Ctr Invest Bionanociencias Elizabeth Jares Erijma, Godoy Cruz 2390, RA-1425 Buenos Aires, DF, Argentina.;Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Organ Analit & Quim, Pabellon 2 Ciudad Univ, RA-1428 Buenos Aires, DF, Argentina..
Consejo Nacl Invest Cient & Tecn, Inst Invest Biomed Buenos Aires IBioBA, Max Planck Soc, Partner Inst, Godoy Cruz 2390, RA-1425 Buenos Aires, DF, Argentina.;Univ Nacl Quilmes, Dept Ciencias & Tecnol, Roque Saenz Pena 352, RA-1876 Bernal, Buenos Aires, Argentina..
KTH, Centres, Science for Life Laboratory, SciLifeLab. Consejo Nacl Invest Cient & Tecn, Ctr Invest Bionanociencias Elizabeth Jares Erijma, Godoy Cruz 2390, RA-1425 Buenos Aires, DF, Argentina.;Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Fis, Pabellon 1 Ciudad Univ, RA-1428 Buenos Aires, DF, Argentina.
Consejo Nacl Invest Cient & Tecn, Ctr Invest Bionanociencias Elizabeth Jares Erijma, Godoy Cruz 2390, RA-1425 Buenos Aires, DF, Argentina.;Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Fis, Pabellon 1 Ciudad Univ, RA-1428 Buenos Aires, DF, Argentina..ORCID iD: 0000-0002-3277-7215
Show others and affiliations
2018 (English)In: Physical Chemistry, Chemical Physics - PCCP, ISSN 1463-9076, E-ISSN 1463-9084, Vol. 10, no 46, p. 29212-29220Article in journal (Refereed) Published
Abstract [en]

Class B G protein-coupled receptors (GPCRs) are involved in a variety of human pathophysiological states. These groups of membrane receptors are less studied than class A GPCRs due to the lack of structural information, delayed small molecule drug discovery, and scarce fluorescence detection tools available. The class B corticotropin-releasing hormone type 1 receptor (CRHR1) is a key player in the stress response whose dysregulation is critically involved in stress-related disorders: psychiatric conditions (i.e. depression, anxiety, and addictions), neuroendocrinological alterations, and neurodegenerative diseases. Here, we present a strategy to label GPCRs with a small fluorescent antagonist that permits the observation of the receptor in live cells through stochastic optical reconstruction microscopy (STORM) with 23 nm resolution. The marker, an aza-BODIPY derivative, was designed based on computational docking studies, then synthesized, and finally tested in biological cells. Experiments on hippocampal neurons demonstrate antagonist effects in similar concentrations as the well-established antagonist CP-376395. A quantitative analysis of two color STORM images enabled the determination of the binding affinity of the new marker in the cellular environment.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2018. Vol. 10, no 46, p. 29212-29220
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-240714DOI: 10.1039/c8cp06196cISI: 000453935400021PubMedID: 30427333Scopus ID: 2-s2.0-85057271533OAI: oai:DiVA.org:kth-240714DiVA, id: diva2:1274995
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20190104

Available from: 2019-01-04 Created: 2019-01-04 Last updated: 2019-01-07Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Barabas, Federico M.Stefani, Fernando D.Cavasotto, Claudio N.Aramendia, Pedro F.
By organisation
Science for Life Laboratory, SciLifeLab
In the same journal
Physical Chemistry, Chemical Physics - PCCP
Chemical Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 20 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf