An improved methodology for the quantification of uronic acid units in xylans and other polysaccharides
2007 (English)In: Carbohydrate Research, ISSN 0008-6215, E-ISSN 1873-426X, Vol. 342, no 11, 1442-1449 p.Article in journal (Refereed) Published
Uronic acids can be quantified either by a colorimetric determination after treatment with concentrated sulfuric acid and carbazole or by gas chromatography after methanolysis and subsequent acetylation. Both methods suffer from incomplete hydrolysis, an unavoidable degradation of the products to be analysed, and an inability to separate and quantify different types of uronic acids. In the present work, the fundamental chemistry involved in the two methods has been evaluated, and some modifications to increase their accuracy are suggested. By combining the two methods, a complete quantification of all individual types of urome acids present in a sample can be achieved.
Place, publisher, year, edition, pages
2007. Vol. 342, no 11, 1442-1449 p.
uronic acid; carbazole; methanolysis; 4-O-methylglucuronic acid; galacturonic acid; glucuronic acid; hexenuronic acid; xylan
Paper, Pulp and Fiber Technology
IdentifiersURN: urn:nbn:se:kth:diva-7714DOI: 10.1016/j.carres.2007.03.031ISI: 000248370800007ScopusID: 2-s2.0-34250650112OAI: oai:DiVA.org:kth-7714DiVA: diva2:12820
QC 201007022007-11-232007-11-232010-07-02Bibliographically approved