Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Investigations on the Interaction of Mucins and Spermatozoa with Chitosan to form Biological Barriers Functioning as a Non-Hormonal Contraceptive
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH).
2019 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Currently, there is a worldwide need for non-hormonal contraceptives, as many women suffer from hormone-related side effects. Pain, bleeding, mood swings, and weightgain, as well as the risk of venous thromboembolism and cervix adenocarcinoma, are only some of the adverse effects that these women have come to accept as a part of their daily lives. This master thesis project is focused on investigating the possibilityof creating a non-hormonal contraceptive by crosslinking cervical mucus to prevent the passage of spermatozoa. For this task, the versatile polysaccharide chitosan was chosen. A biocompatible biopolymer with mucoadhesive properties, which enables the crosslinking of mucins and subsequently strengthens the cervical mucus network. Creating a mechanical barrier that immobilizes the sperm without damaging them.To use chitosan as a contraceptive it is desired to introduce it into a biological system. Therefore, it is of importance to understand the interaction of chitosan during human intercourse. This study focused on investigating the chitosan interaction with mucus and spermatozoa. The study’s four main objectives were to: 1) investigate thecomplexation between different types of chitosans and a model mucin, pig gastric mucin(PGM), 2) investigate the accumulation and penetration of chitosan into cervical mucus,3) investigate whether the addition of excipients to the chitosan formulation changes the chitosan accumulation and diffusion distance in cervical mucus and 4) investigate whether chitosan shows signs of cytotoxicity towards spermatozoa. Complexation of chitosan with PGM was quantified by fluorescence spectroscopy, comparing samples with and without addition of mucins. The accumulation and diffusion distance of chitosanswere investigated by fluorescence microscopy of labeled chitosan diffusing in capillaries filled with cervical mucus. Chitosans’ cytotoxicity analysis was divided into three assays,investigating spermatozoa membrane integrity, Reactive Oxygen Species production andDNA-damage.

Abstract [sv]

För tillfället existerar ett globalt behov av hormonfria preventivmedel. Kvinnor lider och accepterar hormonrelaterade biverkningar så som smärta, blödningar, humörsvängningar,viktökningar och ven trombos. Detta examensarbete fokuserar på forskning och utveckling av ett nytt hormonfritt preventivmedel, som agerar genom att molekylärt tvärbinda kvinnans livmoderhalssekret och förhindra diffusion av spermier. Den biokompatibla polysackariden kitosan valdes för dess förmåga att binda muciner i sekret och stärka det molekylära nätverket. Detta skapar en mekanisk barriär som immobiliserar spermierna utan att skadegöra de.För att använda kitosan som ett preventivmedel är det önskvärt att introducera det i ett biologiskt system. Denna studie fokuserade på att undersöka chitosan-interaktionen med slem och spermatozoa. Studiens fyra huvudmål var följande: 1) Vidareundersöka bildningen mellan olika typer av kitosan och en modell mucin, grismagsmucin (PGM) 2) Vidare undersöka vilken typ av kitosan som ackumuleras mest och tränger längst in i livmoderhalssekret och 3) om tillsats av förtjockningsmedeländrar mängden ackumulation och diffusionsavståndet för kitosan och 4) undersöka om kitosan visar tecken på cytotoxicitet mot spermier. Bindningen av kitosan med PGM kvantifierades med fluorescensspektroskopi genom jämförelse av prover med och utan tillsats av muciner. Kitosans ackumulering och diffusionsavstånd undersöktes med fluorescensmikroskopi och fluorescens märkta kitosan som fördes in i kapillärer fyllda med livmoderhalssekret. Kitosans cytotoxicitet delades in i tre analyser, undersökandet av spermiers membranintegritet, ROS-produktion och skador i DNA:t.

Place, publisher, year, edition, pages
2019.
Keywords [en]
Chitosan, cervical mucus, spermatozoa, non-hormonal contraceptive, fluorescence microscopy, membrane integrity, ROS, DNA
National Category
Natural Sciences Chemical Sciences Engineering and Technology
Identifiers
URN: urn:nbn:se:kth:diva-241592OAI: oai:DiVA.org:kth-241592DiVA, id: diva2:1282178
Available from: 2019-01-24 Created: 2019-01-24 Last updated: 2019-01-24Bibliographically approved

Open Access in DiVA

No full text in DiVA

By organisation
School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH)
Natural SciencesChemical SciencesEngineering and Technology

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 108 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf