Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo
KTH, School of Engineering Sciences (SCI), Applied Physics. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0002-9596-5125
Ist Italiano Tecnol, Mol Microscopy & Spect, Via Morego 30, I-16136 Genoa, Italy..
KTH, Centres, Science for Life Laboratory, SciLifeLab. KTH, School of Engineering Sciences (SCI), Applied Physics.
PSL Res Univ, CNRS, Inst Curie, Lab Phys Chim Curie, F-75005 Paris, France.;Sorbonne Univ, F-75005 Paris, France.;Univ Paris 05, F-75005 Paris, France..
Show others and affiliations
2019 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 10, article id 556Article in journal (Refereed) Published
Abstract [en]

RESOLFT fluorescence nanoscopy can nowadays image details far beyond the diffraction limit. However, signal to noise ratio (SNR) and temporal resolution are still a concern, especially deep inside living cells and organisms. In this work, we developed a non-deterministic scanning approach based on a real-time feedback system which speeds up the acquisition up to 6-fold and decreases the light dose by 70-90% for in vivo imaging. Also, we extended the information content of the images by acquiring the complete temporal evolution of the fluorescence generated by reversible switchable fluorescent proteins. This generates a series of images with different spatial resolution and SNR, from conventional to RESOLFT images, which combined through a multi-image deconvolution algorithm further enhances the effective resolution. We reported nanoscale imaging of organelles up to 35 Hz and actin dynamics during an invasion process at a depth of 20-30 mu m inside a living Caenorhabditis elegans worm.

Place, publisher, year, edition, pages
NATURE PUBLISHING GROUP , 2019. Vol. 10, article id 556
National Category
Physical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-244106DOI: 10.1038/s41467-019-08442-4ISI: 000457443400001PubMedID: 30710076Scopus ID: 2-s2.0-85060941002OAI: oai:DiVA.org:kth-244106DiVA, id: diva2:1289766
Funder
Swedish Research Council
Note

QC 20190219

Available from: 2019-02-19 Created: 2019-02-19 Last updated: 2019-02-19Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Dreier, JesCoceano, GiovannaTesta, Ilaria

Search in DiVA

By author/editor
Dreier, JesCoceano, GiovannaVicidomini, GiuseppeTesta, Ilaria
By organisation
Applied PhysicsScience for Life Laboratory, SciLifeLab
In the same journal
Nature Communications
Physical Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 29 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf