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A Structural Basis for Xe-129 Hyper-CEST Signal in TEM-1 beta-Lactamase
Univ Penn, Dept Chem, 231 S 34th St, Philadelphia, PA 19104 USA..ORCID iD: 0000-0003-3333-5053
Univ Penn, Dept Chem, 231 S 34th St, Philadelphia, PA 19104 USA..
Harvard Med Sch, 300 Longwood Ave, Boston, MA 02115 USA..
KTH, Centres, Science for Life Laboratory, SciLifeLab. KTH, School of Engineering Sciences (SCI), Applied Physics, Biophysics.
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2019 (English)In: ChemPhysChem, ISSN 1439-4235, E-ISSN 1439-7641, Vol. 20, no 2, p. 260-267Article in journal (Refereed) Published
Abstract [en]

Genetically encoded (GE) contrast agents detectable by magnetic resonance imaging (MRI) enable non-invasive visualization of gene expression and cell proliferation at virtually unlimited penetration depths. Using hyperpolarized Xe-129 in combination with chemical exchange saturation transfer, an MR contrast approach known as hyper-CEST, enables ultrasensitive protein detection and biomolecular imaging. GE MRI contrast agents developed to date include nanoscale proteinaceous gas vesicles as well as the monomeric bacterial proteins TEM-1 beta-lactamase (bla) and maltose binding protein (MBP). To improve understanding of hyper-CEST NMR with proteins, structural and computational studies were performed to further characterize the Xe-bla interaction. X-ray crystallography validated the location of a high-occupancy Xe binding site predicted by MD simulations, and mutagenesis experiments confirmed this Xe site as the origin of the observed CEST contrast. Structural studies and MD simulations with representative bla mutants offered additional insight regarding the relationship between local protein structure and CEST contrast.

Place, publisher, year, edition, pages
WILEY-V C H VERLAG GMBH , 2019. Vol. 20, no 2, p. 260-267
Keywords [en]
Xenon, hyperpolarized, CEST, contrast, magnetic resonance
National Category
Biophysics
Identifiers
URN: urn:nbn:se:kth:diva-243952DOI: 10.1002/cphc.201800624ISI: 000456340400010PubMedID: 30151973Scopus ID: 2-s2.0-85053449370OAI: oai:DiVA.org:kth-243952DiVA, id: diva2:1293893
Note

QC 20190305

Available from: 2019-03-05 Created: 2019-03-05 Last updated: 2019-03-05Bibliographically approved

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Kasimova, Marina A.

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