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White Shark Protein Metabolism may be a Model to Improve the Outcome of Cytotoxic Brain Tissue Edema and Cognitive Deficiency after Traumatic Brain Injury and Stroke
Section of Neurosurgery, Karolinska University Hospital and Section of Research, MIPS Company.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Biomedical Engineering and Health Systems, Structural Biotechnology. Department of Biosciences and Nutrition, Karolinska Institute and School of Engineering Sciences in Chemistry, Biotechnology and Health.
Section of Research, MIPS Company.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH). Department of Biosciences and Nutrition, Karolinska Institute and School of Engineering Sciences in Chemistry, Biotechnology and Health.
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2018 (English)In: Journal of Neurology and Neurobiology, ISSN 2379-7150, Vol. 4, no 2Article in journal (Refereed) Published
Abstract [en]

Increased intracellular water content defined as cytotoxic brain tissue edema is a serious secondary clinical complication to traumatic brain injury (TBI) and stroke and without knowledge to the etiology. Recently a hypothesis to the nervous tissue edema was presented suggesting that external dynamic and internal mechanical static impact forces caused protein unfolding resulting in an increased brain tissue water content. The hypothesis was confirmed by computer simulation tests. In this laboratory study we further evaluated the hypothesis by using the mature protein laminin LN521 upon the effects of both dynamic as well as static impact forces, respectively. Laminin was chosen as a representative protein due to it´s general and abundance presence in the cells. The treated laminin solutions were then analyzed with denatured electrophoresis and Electron Microscopy showing aggregation and fragmentation of the laminin structures. The present results confirm earlier hypothesis and computer simulation suggesting for the first time that dynamic impact force in an accident and increased mechanical static force in stroke unfold mature proteins having the potential to increase the intracellular water content defined as cytotoxic brain tissue edema. The clinical condition resembles the phenomenon when elasmobranchs including white sharks prevent their cells from too high hydrostatic pressure in the deep sea. Thus, the present laboratory study results and knowledge from marine physics may be considered to improve the clinical treatment and outcome of TBI and stroke patients.

Place, publisher, year, edition, pages
2018. Vol. 4, no 2
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Medical and Health Sciences
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URN: urn:nbn:se:kth:diva-248785DOI: 10.16966/2379-7150.151OAI: oai:DiVA.org:kth-248785DiVA, id: diva2:1303611
Note

QC 20190627

Available from: 2019-04-10 Created: 2019-04-10 Last updated: 2019-08-29Bibliographically approved

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Purhonen, PasiHebert, Hans

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