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Fermentation of glucose-xylose-arabinose mixtures by a synthetic consortium of single-sugar-fermenting Saccharomyces cerevisiae strains
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Industrial Biotechnology. Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, Delft, 2629, Netherlands.
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2018 (English)In: FEMS yeast research (Print), ISSN 1567-1356, E-ISSN 1567-1364, Vol. 18, no 8, article id foy075Article in journal (Refereed) Published
Abstract [en]

D-glucose, D-xylose and L-arabinose are major sugars in lignocellulosic hydrolysates. This study explores fermentation of glucose-xylose-arabinose mixtures by a consortium of three 'specialist' Saccharomyces cerevisiae strains. A D-glucose- and L-arabinose-tolerant xylose specialist was constructed by eliminating hexose phosphorylation in an engineered xylose-fermenting strain and subsequent laboratory evolution. A resulting strain anaerobically grew and fermented D-xylose in the presence of 20 g L-1 of D-glucose and L-arabinose. A synthetic consortium that additionally comprised a similarly obtained arabinose specialist and a pentose non-fermenting laboratory strain, rapidly and simultaneously converted D-glucose and L-arabinose in anaerobic batch cultures on three-sugar mixtures. However, performance of the xylose specialist was strongly impaired in these mixed cultures. After prolonged cultivation of the consortium on three-sugar mixtures, the time required for complete sugar conversion approached that of a previously constructed and evolved 'generalist' strain. In contrast to the generalist strain, whose fermentation kinetics deteriorated during prolonged repeated-batch cultivation on a mixture of 20 g L-1 D-glucose, 10 g L-1 D-xylose and 5 g L-1 L-arabinose, the evolved consortium showed stable fermentation kinetics. Understanding the interactions between specialist strains is a key challenge in further exploring the applicability of this synthetic consortium approach for industrial fermentation of lignocellulosic hydrolysates.

Place, publisher, year, edition, pages
Oxford University Press, 2018. Vol. 18, no 8, article id foy075
Keywords [en]
synthetic consortium, division of labour, evolutionary engineering, pentose fermentation, bioethanol, yeast
National Category
Industrial Biotechnology
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URN: urn:nbn:se:kth:diva-249352ISI: 000456030700002PubMedID: 30010916Scopus ID: 2-s2.0-85055076960OAI: oai:DiVA.org:kth-249352DiVA, id: diva2:1304202
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QC 20190625

Available from: 2019-04-11 Created: 2019-04-11 Last updated: 2019-06-25Bibliographically approved

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