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Collection and X-ray microanalysis of airway surface liquid in the mouse using ion exchange beads.
2004 (English)In: Micron, ISSN 0968-4328, E-ISSN 1878-4291, Vol. 35, no 8, p. 701-5Article in journal (Refereed) Published
Abstract [en]

The airway surface liquid (ASL) is a thin layer of liquid covering the airway epithelium. The ionic composition of the ASL is assumed to be important for airway function and may be altered in diseases such as cystic fibrosis and exercise-induced asthma. A method for collection of ASL is presented in which the fluid is collected using small dextran ion-exchange beads. The beads are equilibrated with the ASL in a humidity chamber, collected under silicon oil, dried and analyzed by X-ray microanalysis. Analysis of standard beads prepared by exposure to different salt solutions shows that linear calibration lines can be obtained, but that beads absorb different elements to a different extent. The results show that the ASL in mice is hypotonic, and that the mucus component of the ASL has an elemental composition that is different from that of the periciliary fluid.

Place, publisher, year, edition, pages
2004. Vol. 35, no 8, p. 701-5
National Category
Natural Sciences Medical and Health Sciences
Identifiers
URN: urn:nbn:se:kth:diva-249490DOI: 10.1016/j.micron.2004.03.005PubMedID: 15288649OAI: oai:DiVA.org:kth-249490DiVA, id: diva2:1304485
Note

QC 20190412

Available from: 2019-04-12 Created: 2019-04-12 Last updated: 2019-04-12Bibliographically approved

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