Transcription factor concentrations versus binding site affinities in the yeast S. cerevisiae
2007 (English)In: Physical Biology, ISSN 1478-3975, Vol. 4, no 2, 134-143 p.Article in journal (Refereed) Published
Transcription regulation is largely governed by the profile and the dynamics of transcription factors' binding to DNA. Stochastic effects are intrinsic to this dynamics, and the binding to functional sites must be controlled with a certain specificity for living organisms to be able to elicit specific cellular responses. Specificity stems here from the interplay between binding affinity and cellular abundance of transcription factor proteins, and the binding of such proteins to DNA is thus controlled by their chemical potential. We combine large-scale protein abundance data in the budding yeast with binding affinities for all transcription factors with known DNA binding site sequences to assess the behavior of their chemical potentials in an exponential growth phase. A sizable fraction of transcription factors is apparently bound non-specifically to DNA, and the observed abundances are marginally sufficient to ensure high occupations of the functional sites. We argue that a biological cause of this feature is related to its noise-filtering consequences: abundances below physiological levels do not yield significant binding of functional targets and mis-expressions of regulated genes may thus be tamed.
Place, publisher, year, edition, pages
2007. Vol. 4, no 2, 134-143 p.
FACILITATED TARGET LOCATION, GENE-REGULATION, NETWORK MOTIFS, REGULATORY NETWORK, ESCHERICHIA-COLI, DNA INTERACTION, SINGLE-CELL, PROTEIN, EXPRESSION, NUMBERS
Condensed Matter Physics
IdentifiersURN: urn:nbn:se:kth:diva-7972DOI: 10.1088/1478-3975/4/2/006ISI: 000247779500007ScopusID: 2-s2.0-34447279073OAI: oai:DiVA.org:kth-7972DiVA: diva2:13166
QC 20101005. Tidigare titel:"Transcription factor concentrations versus binding site affinities in the yeast S. cerevisiae. Numbers and affinity".2008-02-122008-02-122011-03-17Bibliographically approved