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Wood-based nanocellulose and bioactive glass modified gelatin-alginate bioinks for 3D bioprinting of bone cells
Tampere Univ, Fac Med & Hlth Technol, Adult Stem Cell Res Grp, Tampere, Finland.;Tampere Univ Hosp, Res Dev & Innovat Ctr, Tampere, Finland.;Univ Bergen, Fac Med, Dept Clin Dent, Tissue Engn Grp, Bergen, Norway..ORCID iD: 0000-0002-5493-3530
Univ Bergen, Fac Med, Dept Clin Dent, Tissue Engn Grp, Bergen, Norway..
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Fibre- and Polymer Technology.
Tampere Univ, Fac Med & Hlth Technol, Lab Biomat & Tissue Engn, Tampere, Finland..ORCID iD: 0000-0002-1099-8420
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2019 (English)In: Biofabrication, ISSN 1758-5082, E-ISSN 1758-5090, Vol. 11, no 3, article id 035010Article in journal (Refereed) Published
Abstract [en]

A challenge in the extrusion-based bioprinting is to find a bioink with optimal biological and physicochemical properties. The aim of this study was to evaluate the influence of wood-based cellulose nanofibrils (CNF) and bioactive glass (BaG) on the rheological properties of gelatin-alginate bioinks and the initial responses ofbone cells embedded in these inks. CNF modulated the flow behavior of the hydrogels, thus improving their printability. Chemical characterization by SEM-EDX and ion release analysis confirmed the reactivity of the BaG in the hydrogels. The cytocompatibility of the hydrogels was shown to be good, as evidenced by the viability of human osteoblast-like cells (Saos-2) in cast hydrogels. For bioprinting, 4-layer structures were printed from cell-containing gels and crosslinked with CaCl2. Viability, proliferation and alkaline phosphatase activity (ALP) were monitored over 14 d. In the BaG-free gels, Saos-2 cells remained viable, but in the presence of BaG the viability and proliferation decreased in correlation with the increased viscosity. Still, there was a constant increase in the ALP activity in all the hydrogels. Further bioprinting experiments were conducted using human bone marrow-derived mesenchymal stem cells (hBMSCs), a clinically relevant cell type. Interestingly, hBMSCs tolerated the printing process better than Saos-2 cells and the ALP indicated BaG-stimulated early osteogenic commitment. The addition of CNF and BaG to gelatin-alginate bioinks holds great potential for bone tissue engineering applications.

Place, publisher, year, edition, pages
Institute of Physics Publishing (IOPP), 2019. Vol. 11, no 3, article id 035010
Keywords [en]
bioprinting, viscosity, Saos-2, mesenchymal stem cell, bone tissue engineering, bioink, cellulose nanofibril
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-251692DOI: 10.1088/1758-5090/ab0692ISI: 000466273600004PubMedID: 30754034Scopus ID: 2-s2.0-85063390553OAI: oai:DiVA.org:kth-251692DiVA, id: diva2:1316639
Note

QC 20190520

Available from: 2019-05-20 Created: 2019-05-20 Last updated: 2019-07-29Bibliographically approved

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Ahlinder, AstridFinne Wistrand, Anna

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