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In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
Karolinska Inst, Stockholm, Sweden.;Sci Life Lab, Proteogen, Solna, Sweden..ORCID iD: 0000-0003-4624-031X
Karolinska Inst, Stockholm, Sweden..
Karolinska Inst, Stockholm, Sweden.;Sci Life Lab, Proteogen, Solna, Sweden..
Karolinska Inst, Stockholm, Sweden.;Sci Life Lab, Proteogen, Solna, Sweden..
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2019 (English)In: eLIFE, E-ISSN 2050-084X, Vol. 8, article id e41608Article in journal (Refereed) Published
Abstract [en]

Here, we present a method for in-depth human plasma proteome analysis based on high-resolution isoelectric focusing HiRIEF LC-MS/MS, demonstrating high proteome coverage, reproducibility and the potential for liquid biopsy protein profiling. By integrating genomic sequence information to the MS-based plasma proteome analysis, we enable detection of single amino acid variants and for the first time demonstrate transfer of multiple protein variants between mother and fetus across the placenta. We further show that our method has the ability to detect both low abundance tissue-annotated proteins and phosphorylated proteins in plasma, as well as quantitate differences in plasma proteomes between the mother and the newborn as well as changes related to pregnancy.

Place, publisher, year, edition, pages
ELIFE SCIENCES PUBLICATIONS LTD , 2019. Vol. 8, article id e41608
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Biological Sciences
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URN: urn:nbn:se:kth:diva-252639DOI: 10.7554/eLife.41608ISI: 000468060900001PubMedID: 30958262Scopus ID: 2-s2.0-85066163570OAI: oai:DiVA.org:kth-252639DiVA, id: diva2:1321913
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QC 20190610

Available from: 2019-06-10 Created: 2019-06-10 Last updated: 2019-06-10Bibliographically approved

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Schwenk, Jochen M.

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