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Normal and Friction Forces between Mucin and Mucin-Chitosan Layers in Absence and Presence of SDS
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface Chemistry.ORCID iD: 0000-0002-5444-7276
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface Chemistry.ORCID iD: 0000-0002-2288-819X
2008 (English)In: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 324, no 1-2, 246-256 p.Article in journal (Refereed) Published
Abstract [en]

Employing the colloidal probe AFM technique we have investigated normal and friction forces between flat mica surfaces and silica particles coated with mucin and combined mucin-chitosan layers in presence and absence of anionic surfactant, SDS, in 30 mM NaCl solution. We have shown that the normal interactions between mucin coated mica and silica surfaces are dominated by long-range steric repulsion on both compression and decompression. Friction forces between such mucin layers are characterized by a low effective friction coefficient, mu(eff) = 0.03 +/- 0.02, which is lower than the value of 0.13 +/- 0.02 observed when chitosan layers were adsorbed. Forces between combined mucin-chitosan layers have Adsorption of chitosan on mucin results in considerable compaction of the layer, also been measured. and development of attractive forces detectable on separation. Friction between mucin-chitosan layers in 30 mM NaCl solution is high, with mu(eff) approximate to 0.4. Adsorption of additional mucin to this layer results in no improvement with respect to lubrication as compared to the mucin-chitosan layer, and mu(eff) approximate to 0.4 is We argue that the layers containing both mucin and chitosan are not strictly layered but rather observed. As a result attractive interactions between oppositely charged moieties of sialic acid strongly entangled. residues from mucin and amine groups from chitosan residing on the opposing surfaces contribute to the increased friction. The effects of SDS on normal and friction forces between combined mucin-chitosan layers were also investigated. The relation between surface interactions and friction properties is discussed.

Place, publisher, year, edition, pages
2008. Vol. 324, no 1-2, 246-256 p.
Keyword [en]
mucin, chitosan, SDS, friction force, AFM, lubrication, steric force, adhesion
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-8078DOI: 10.1016/j.jcis.2008.04.055ISI: 000257232100037Scopus ID: 2-s2.0-49949152619OAI: oai:DiVA.org:kth-8078DiVA: diva2:13304
Note
QC 20100903. Uppdaterad från submitted till published (20100903).Available from: 2008-03-07 Created: 2008-03-07 Last updated: 2010-09-03Bibliographically approved
In thesis
1. Lubrication and Surface Properties of Adsorbed Layers of Polyelectrolytes and Proteins
Open this publication in new window or tab >>Lubrication and Surface Properties of Adsorbed Layers of Polyelectrolytes and Proteins
2008 (English)Doctoral thesis, comprehensive summary (Other scientific)
Abstract [en]

Friction forces between protein / polyelectrolyte layers, adsorption properties of proteins, and conformational changes due to variation in electrolyte concentration have been investigated. The aim was to obtain better understanding of adsorbed layer properties, with focus on the relation between layer structure and lubrication capabilities. The major techniques used were AFM (atomic force microscope) with colloidal probe for normal force and friction measurements together with QCM-D (quartz crystal microbalance with dissipation) for measurement of adsorption and conformational changes of adsorbed layers.

A comparison between some techniques for calibration of the AFM instrument for friction measurements was made to find the most suitably one for colloidal probe friction measurements in aqueous solutions. It is suggested that the normal and torsional Sader methods are preferred in combination with torsional detector sensitivity measurement, for which one new methodology has been proposed.

Adsorption was studied for bovine serum albumin, cytochrome c, myoglobin and mucin, whereas conformational changes of the adsorbed layer were monitored only for mucin. It was found that it was essential to take into account bulk density and viscosity changes for correct interpretations of QCM data when studying the effect of changes in electrolyte type and concentration on preadsorbed layers of mucin, and also when having different (high) concentrations of proteins in the measuring solution. The adsorbed amount of proteins appears to depend on the strength of the surface attachment, in such a manner that a too high affinity reduces the adsorbed amount.

Friction properties in aqueous solution have been studied for adsorbed layers of PEO45MEMA:METAC co-polyelectrolytes, with varying density of grafted PEO45 side chains and varying charge density, as well as for a naturally occurring polyelectrolyte (chitosan) and the glycoprotein mucin. These polymers were used to cover a wide range of different types of adsorbed layers and interactions to gain a better understanding of friction mechanisms and demands on layer properties for achieving favourable lubrication. It was found that the common features of low friction layers are that no attractive forces are present, and that excluded volume and / or electrostatic forces counteract chain interpenetration under load.

Place, publisher, year, edition, pages
Stockholm: KTH, 2008. vii, 59 p.
Series
Trita-CHE-Report, ISSN 1654-1081 ; 2008:16
Keyword
biochemistry, biomedical engineering
National Category
Physical Chemistry
Identifiers
urn:nbn:se:kth:diva-4663 (URN)978-91-7178-891-7 (ISBN)
Public defence
2008-03-27, Sal F3, KTH, Lindstedtsvägen 26, Stockholm, 10:00
Opponent
Supervisors
Note
QC 20100903Available from: 2008-03-07 Created: 2008-03-07 Last updated: 2010-09-03Bibliographically approved

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Pettersson, TorbjörnDėdinaitė, Andra

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