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Processability work-flow for assessment of SLE affimAbs
KTH, School of Engineering Sciences (SCI), Applied Physics.
2019 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Systemic Lupus Erythematosus (SLE) is an autoimmune in ammatory disease causing tissuedamage in organs and can eventually be lethal. The immunopathogenesis of SLE is complexand production of bi-specic fusion proteins (amAbs) inhibiting the activity of both IFN-and C5 is one hope to cure the disease. Due to high cost related to drug development, a work ow for quick high throughput screening of stability and solubility of possible drug candidatesis desirable. Through rigorous testing, an optimized work- ow was devised. This work- owis described, tested on 10 amAbs and has proven to be an alternative that requires 600 gprotein which is 80% less protein needed than for an unoptimized work ow. The constructsexamined in this report varies with respect to antibody subtype and fusion point, where IgG1and HC-fusion appear to have higher stability. The thermal stability study suggests that thelead candidates are Construct 2 and 7 and ought to be included in the continuous developmentof SLE amAbs.

Place, publisher, year, edition, pages
2019.
Series
TRITA-SCI-GRU ; 2019:218
National Category
Physical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-254433OAI: oai:DiVA.org:kth-254433DiVA, id: diva2:1332273
Available from: 2019-06-28 Created: 2019-06-28 Last updated: 2019-06-28Bibliographically approved

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