The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II
2006 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 103, no 43, 15788-15793 p.Article in journal (Refereed) Published
CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.
Place, publisher, year, edition, pages
2006. Vol. 103, no 43, 15788-15793 p.
electron microscopy; transcription; protein structure; yeast; Schizosaccharomyces pombe
Natural Sciences Natural Sciences
IdentifiersURN: urn:nbn:se:kth:diva-8114DOI: 10.1073/pnas.0607483103ISI: 000241568500013ScopusID: 2-s2.0-33750481873OAI: oai:DiVA.org:kth-8114DiVA: diva2:13346
QC 201007192008-03-182008-03-182010-10-01Bibliographically approved