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Heterobasidion-growth inhibiting Bacillus subtilis A18 exhibits medium- and age-dependent production of lipopeptides
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry. Department of Chemistry, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, Pakistan.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Industrial Biotechnology.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Organic chemistry. Division of Organic Chemistry, Institute of Technology, Tartu University, Tartu 50411, Estonia.
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2019 (English)In: Microbiology Research, ISSN 0944-5013, E-ISSN 1618-0623, Vol. 223-225, p. 129-136Article in journal (Refereed) Published
Abstract [en]

Heterobasidion annosum s.s. and H. parviporum are severe pathogens of conifers causing butt rot and root rot thus reducing the economic value of timber. Here, the antifungal activity of Bacillus subtilis isolate A18 against these two Heterobasidion species was investigated. Five different culture media with different culture age were investigated to study the effect of substrate composition and culture age for metabolite production. Bacterial cultures and cell-free culture filtrates were tested for antifungal activity. Inhibition of fungal growth was analysed using the agar disc-diffusion method. MALDI-TOF and LC-HRMS analyses were used to identify the antifungal metabolites. Substrate composition and age of culture were found to be active variables with direct effect on the antifungal activity of bacterial culture extracts. High anti-fungal activity was observed when B. subtilis was cultured in PDB, SGB and LB media for four days. Mass-spectrometry analysis showed the presence of lipopeptides in culture filtrates identified as members of the surfactins, polymixins, kurstakins and fengycins. A culture filtrate containing fengycin-type lipopeptides showed the highest bioactivity against Heterobasidion species. Bacterial cultures had higher bioactivity compared to their respective cell free culture filtrates. The results of the present study suggest that B. subtilis A18 is a powerful biocontrol agent against Heterobasidion infections of tree wounds and stumps.

Place, publisher, year, edition, pages
Elsevier GmbH , 2019. Vol. 223-225, p. 129-136
Keywords [en]
Anti-fungal compounds, Biocontrol, Co-culturing, Conifers, Inhibition, Lipopeptides, Bacteria, Bacteriology, Enzyme inhibition, Forestry, Mass spectrometry, Metabolites, Substrates, Antifungal compounds, Antifungal metabolite, Heterobasidion annosum, Mass spectrometry analysis, Metabolite production, Substrate composition, Fungi, Bacillus subtilis, Bacteria (microorganisms), Coniferophyta, Heterobasidion, Heterobasidion parviporum
National Category
Organic Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-252448DOI: 10.1016/j.micres.2019.04.006Scopus ID: 2-s2.0-85065092748OAI: oai:DiVA.org:kth-252448DiVA, id: diva2:1337492
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QC 20190715

Available from: 2019-07-15 Created: 2019-07-15 Last updated: 2019-07-15Bibliographically approved

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Azeem, MuhammadBorg-Karlson, Anna-KarinRajarao, Gunaratna Kuttuva

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