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QCM sensing of multivalent interactions between lectins and well-defined glycosylated nanoplatforms
Univ Namur, Dept Chim, Lab Chim Bioorgan, Rue Bruxelles 61, B-5000 Namur, Belgium.;PSL Univ, CNRS, ESPCI Paris, Mol Macromol Chem & Mat, 10 Rue Vauquelin, F-75005 Paris, France..
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry.
Attana AB, Bjornnasvagen 21, S-11419 Stockholm, Sweden..
Attana AB, Bjornnasvagen 21, S-11419 Stockholm, Sweden..
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2019 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 139, article id 111328Article in journal (Refereed) Published
Abstract [en]

Quartz crystal microbalance (QCM) methodology has been adopted to unravel important factors contributing to the "cluster glycoside effect" observed in carbohydrate-lectin interactions. Well-defined, glycosylated nanostructures of precise sizes, geometries and functionalization patterns were designed and synthesized, and applied to analysis of the interaction kinetics and thermodynamics with immobilized lectins. The nanostructures were based on Borromean rings, dodecaamine cages, and fullerenes, each of which carrying a defined number of carbohydrate ligands at precise locations. The synthesis of the Borromeates and dodecaamine cages was easily adjustable due to the modular assembly of the structures, resulting in variations in presentation mode. The binding properties of the glycosylated nanoplatforms were evaluated using flow-through QCM technology, as well as hemagglutination inhibition assays, and compared with dodecaglycosylated fullerenes and a monovalent reference. With the QCM setup, the association and dissociation rate constants and the associated equilibrium constants of the interactions could be estimated, and the results used to delineate the multivalency effects of the lectin-nanostructure interactions.

Place, publisher, year, edition, pages
Elsevier, 2019. Vol. 139, article id 111328
Keywords [en]
QCM, Carbohydrates, Lectins, Multivalency, Nanoplatforms
National Category
Organic Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-255296DOI: 10.1016/j.bios.2019.111328ISI: 000472696000013PubMedID: 31136921Scopus ID: 2-s2.0-85066129421OAI: oai:DiVA.org:kth-255296DiVA, id: diva2:1339537
Note

QC 20190730

Available from: 2019-07-30 Created: 2019-07-30 Last updated: 2019-12-20Bibliographically approved

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Ramström, Olof

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