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Silkworm Silk Matrices Coated with Functionalized Spider Silk Accelerate Healing of Diabetic Wounds
Indian Inst Technol Guwahati, Dept Biosci & Bioengn, Biomat & Tissue Engn Lab, Gauhati 781039, Assam, India..
West Bengal Univ Anim & Fishery Sci, Dept Vet Surg & Radiol, Kolkata 700037, W Bengal, India..
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science.
West Bengal Univ Anim & Fishery Sci, Dept Vet Surg & Radiol, Kolkata 700037, W Bengal, India..
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2019 (English)In: ACS Biomaterials Science and Engineering, ISSN 2373-9878, Vol. 5, no 7, p. 3537-3548Article in journal (Refereed) Published
Abstract [en]

Complex cutaneous wounds like diabetic foot ulcers represent a critical clinical challenge and demand a large-scale and low-cost strategy for effective treatment. Herein, we use a rabbit animal model to investigate efficacy of bioactive wound dressings made up of silk biomaterials. Nanofibrous mats of Antheraea assama silkworm silk fibroin (AaSF) are coated with various recombinant spider silk fusion proteins through silk-silk interactions to fabricate multifunctional wound dressings. Two different types of spider silk coatings are used to compare their healing efficiency: FN-4RepCT (contains a cell binding motif derived from fibronectin) and Lac-4RepCT (contains a cationic antimicrobial peptide from lactoferricin). AaSF mats coated with spider silk show accelerated wound healing properties in comparison to the uncoated mats. Among the spider silk coated variants, dual coating of FN-4RepCT and Lac-4RepCT on top of AaSF mat demonstrated better wound healing efficiency, followed by FN-4RepCT and Lac-4RepCT single coated counterparts. The in vivo study also reveals excellent skin regeneration by the functionalized silk dressings in comparison to commercially used Duoderm dressing and untreated wounds. The spider silk coatings demonstrate early granulation tissue development, re-epithelialization, and efficient matrix remodelling of wounds. The results thus validate potential of bioactive silk matrices in faster repair of diabetic wounds.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2019. Vol. 5, no 7, p. 3537-3548
Keywords [en]
silk fibroin, recombinant spider silk, nanofibrous mat, wound healing, diabetic wounds
National Category
Biomaterials Science
Identifiers
URN: urn:nbn:se:kth:diva-255569DOI: 10.1021/acsbiomaterials.9b00514ISI: 000474812300034Scopus ID: 2-s2.0-85068124442OAI: oai:DiVA.org:kth-255569DiVA, id: diva2:1340147
Note

QC 20190802

Available from: 2019-08-02 Created: 2019-08-02 Last updated: 2019-10-04Bibliographically approved

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Thatikonda, NareshHedhammar, My

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