Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Correlative STED super-resolution light and electron microscopy on resin sections
Max Planck Inst Biophys Chem, Dept NanoBiophoton, Gottingen, Germany.;Abberior Instruments GmbH, Gottingen, Germany..
Max Planck Inst Dev Biol, Tubingen, Germany..
KTH, School of Engineering Sciences (SCI), Applied Physics. KTH, Centres, Science for Life Laboratory, SciLifeLab. Max Planck Inst Biophys Chem, Dept NanoBiophoton, Gottingen, Germany.;Univ Med Ctr Gottingen, Clin Neurol, Gottingen, Germany.
Max Planck Inst Biophys Chem, Lab Electron Microscopy, D-37077 Gottingen, Germany..
Show others and affiliations
2019 (English)In: Journal of Physics D: Applied Physics, ISSN 0022-3727, E-ISSN 1361-6463, Vol. 52, no 37, article id 374003Article in journal (Refereed) Published
Abstract [en]

Correlative light and electron microscopy approaches can reveal the localisation of specific proteins while providing detailed information on the cellular context, thereby combining the strengths of both imaging modalities. The major challenge in combining fluorescence microscopy with electron microscopy is the different sample preparation requirements necessary for obtaining high quality data from both modalities. To overcome this limitation, we combined conventional sample preparation protocols for electron microscopy with post-embedding labelling on ultra-thin sections using antibodies and other specific ligands. We successfully employed STED super-resolution microscopy to image the subcellular distribution of several targets in various specimen including E. coli, T brucei, S. cerevisiae, human cancer cells and bovine sperm. Thus, we present widely applicable methods facilitating the use of antibodies for correlative super-resolution light and electron microscopy of post-embedding labelled targets.

Place, publisher, year, edition, pages
Institute of Physics (IOP), 2019. Vol. 52, no 37, article id 374003
Keywords [en]
CLEM, STED, super-resolution microscopy, immunolabelling, immunogold, resin embedding, correlative microscopy
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:kth:diva-255543DOI: 10.1088/1361-6463/ab2b31ISI: 000475773000001OAI: oai:DiVA.org:kth-255543DiVA, id: diva2:1340198
Note

QC 20190802

Available from: 2019-08-02 Created: 2019-08-02 Last updated: 2019-08-02Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full text

Authority records BETA

Jans, Daniel C.

Search in DiVA

By author/editor
Jans, Daniel C.
By organisation
Applied PhysicsScience for Life Laboratory, SciLifeLab
In the same journal
Journal of Physics D: Applied Physics
Biomedical Laboratory Science/Technology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 8 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf