Methodological improvements of pyrosequencing technology
2006 (English)In: Journal of Biotechnology, ISSN 0168-1656, E-ISSN 1873-4863, Vol. 124, no 3, 504-511 p.Article in journal (Refereed) Published
Pyrosequencing technology is a rather novel DNA sequencing method based on the sequencing-by-synthesis principle. This bioluminometric, real-time DNA sequencing technique employs a cascade of four enzymatic reactions producing sequence peak signals. The method has been proven highly suitable for single nucleotide polymorphism analysis and sequencing of short stretches of DNA. Although the pyrosequencing procedure is relatively straightforward, users may face challenges due to varying parameters in PCR and sequencing primer design, sample preparation and nucleotide dispensation; such challenges are labor and cost intensive. In this study, these issues have been addressed to increase signal quality and assure sequence accuracy.
Place, publisher, year, edition, pages
2006. Vol. 124, no 3, 504-511 p.
DNA sequencing; primer-dimers; pyrosequencing technology; sample preparation; single-strand binding protein; SINGLE-NUCLEOTIDE POLYMORPHISM; TUMOR-SUPPRESSOR GENE; STRANDED DNA-BINDING; SEQUENCING PRIMERS; MUTATION; PROTEIN
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-8158DOI: 10.1016/j.jbiotec.2006.01.025ISI: 000239156700004ScopusID: 2-s2.0-33745504382OAI: oai:DiVA.org:kth-8158DiVA: diva2:13407
QC 201006242008-04-032008-04-032013-02-26Bibliographically approved