Sentinel-base DNA genotyping using multiple sequencing primers for high-risk human papillomaviruses
2006 (English)In: Molecular and Cellular Probes, ISSN 0890-8508, E-ISSN 1096-1194, Vol. 20, no 3-4, 230-238 p.Article in journal (Refereed) Published
Despite the various technologies in place for genotyping human papillomaviruses (HPV), clinical use and clinical research demand a method that is fast, more reliable and cost-effective. The technology described here represents a breakthrough development in that direction. By combining the method of multiple sequencing primers with DNA sequencing, we have developed a rapid assay for genotyping HPV that relies on the identification of a single, type-specific 'sentinel' base. As described here, the prototype assay has been developed to recognize the 12 most high-risk HPV types (HPV-16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58 and 59) and is capable of recognizing and simultaneously genotyping multiple HPV co-infections. By providing sequence information on multiple HPV infections, this method eliminates the need for labor- and cost-intensive PCR cloning. These proof-of-concept studies establish the assay to be accurate, reliable, rapid, flexible, and cost-effective, providing evidence of the feasibility this technique for use in clinical settings.
Place, publisher, year, edition, pages
2006. Vol. 20, no 3-4, 230-238 p.
human papillomaviruses (HPV); DNA sequencing; multiple infections; multiple sequencing primers; sentinel-base DNA sequencing; pyrosequencing technology; POLYMERASE-CHAIN-REACTION; GENITAL HUMAN PAPILLOMAVIRUSES; CERVICAL-CANCER; HYBRID CAPTURE; GENERAL PRIMERS; NESTED PCR; SPECIMENS; SAMPLES; HPV; IDENTIFICATION
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-8160DOI: 10.1016/j.mcp.2006.01.002ISI: 000238113600011ScopusID: 2-s2.0-33646252262OAI: oai:DiVA.org:kth-8160DiVA: diva2:13409
QC 201006242008-04-032008-04-032010-06-24Bibliographically approved