Connector Inversion Probe Technology: A Powerful One- Primer Multiplex DNA Amplification System for Numerous Scientific Applications
2007 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 2, no 9, e915- p.Article in journal (Refereed) Published
We combined components of a previous assay referred to as Molecular Inversion Probe (MIP) with a complete gap filling strategy, creating a versatile powerful one-primer multiplex amplification system. As a proof-of-concept, this novel method, which employs a Connector Inversion Probe (CIPer), was tested as a genetic tool for pathogen diagnosis, typing, and antibiotic resistance screening with two distinct systems: i) a conserved sequence primer system for genotyping Human Papillomavirus (HPV), a cancer-associated viral agent and ii) screening for antibiotic resistance mutations in the bacterial pathogen Neisseria gonorrhoeae. We also discuss future applications and advances of the CIPer technology such as integration with digital amplification and next-generation sequencing methods. Furthermore, we introduce the concept of two-dimension informational barcodes, i. e. "multiplex multiplexing padlocks'' (MMPs). For the readers' convenience, we also provide an on-line tutorial with user-interface software application CIP creator 1.0.1, for custom probe generation from virtually any new or established primer-pairs.
Place, publisher, year, edition, pages
2007. Vol. 2, no 9, e915- p.
DNA; DNA Probes; Drug Resistance, Microbial; Mutation
IdentifiersURN: urn:nbn:se:kth:diva-8162DOI: 10.1371/journal.pone.0000915ISI: 000207455700023ScopusID: 2-s2.0-41249094334OAI: oai:DiVA.org:kth-8162DiVA: diva2:13411
QC 201006242008-04-032008-04-032010-06-24Bibliographically approved