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3D Primary Hepatocyte Culture Systems for Analyses of Liver Diseases, Drug Metabolism, and Toxicity: Emerging Culture Paradigms and Applications
Karolinska Inst, Sect Pharmacogenet, Dept Physiol & Pharmacol, Biomedicum 5B, SE-17177 Stockholm, Sweden..
KTH, School of Electrical Engineering and Computer Science (EECS), Micro and Nanosystems.
Department of Physiology and Pharmacology, Section of Pharmacogenetics, Biomedicum 5B, Karolinska Institutet, Stockholm, SE-171 77, Sweden; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Centre (UMC) Utrecht, Utrecht, 3584 CT, Netherlands .
Department of Physiology and Pharmacology, Section of Pharmacogenetics, Biomedicum 5B, Karolinska Institutet, Stockholm, SE-171 77, Sweden.
2019 (English)In: Biotechnology Journal, ISSN 1860-6768, E-ISSN 1860-7314, Vol. 14, no 7, article id 1800347Article, review/survey (Refereed) Published
Abstract [en]

Recent research has shown that the maintenance of relevant liver functions ex vivo requires models in which the cells exhibit an in vivo-like phenotype, often achieved by reconstitution of appropriate cellular interactions. Multiple different models have been presented that differ in the cells utilized, media, and culture conditions. Furthermore, several technologically different approaches have been presented including bioreactors, chips, and plate-based systems in fluidic or static media constituting of chemically diverse materials. Using such models, the ability to predict drug metabolism, drug toxicity, and liver functionality have increased tremendously as compared to conventional in vitro models in which cells are cultured as 2D monolayers. Here, the authors highlight important considerations for microphysiological systems for primary hepatocyte culture, review current culture paradigms, and discuss their opportunities for studies of drug metabolism, hepatotoxicity, liver biology, and disease.

Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2019. Vol. 14, no 7, article id 1800347
Keywords [en]
cytochrome P450, drug metabolism, hepatotoxicity, liver fibrosis, liver-on-a-chip, microfluidic systems, spheroids, steatosis
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-255768DOI: 10.1002/biot.201800347ISI: 000476825900004PubMedID: 30957976Scopus ID: 2-s2.0-85068774957OAI: oai:DiVA.org:kth-255768DiVA, id: diva2:1341544
Note

QC 20190809

Available from: 2019-08-09 Created: 2019-08-09 Last updated: 2019-08-09Bibliographically approved

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