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Efficacy of copolymer scaffolds delivering human demineralised dentine matrix for bone regeneration
Univ Bergen, Ctr Clin Dent Res, Dept Clin Dent, Fac Med, N-5009 Bergen, Norway..
Univ Bergen, Dept Biomed, Bergen, Norway..
Cardiff Univ, Dept Oral & Biomed Sci, Sch Dent, Cardiff, S Glam, Wales.;CITER, Cardiff, S Glam, Wales..
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Fibre- and Polymer Technology.
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2019 (English)In: Journal of Tissue Engineering, ISSN 2041-7314, E-ISSN 2041-7314, Vol. 10, article id 2041731419852703Article in journal (Refereed) Published
Abstract [en]

Poly(L-lactide-co-epsilon-caprolactone) scaffolds were functionalised by 10 or 20 mu g/mL of human demineralised dentine matrix. Release kinetics up to 21 days and their osteogenic potential on human bone marrow stromal cells after 7 and 21 days were studied. A total of 390 proteins were identified by mass spectrometry. Bone regeneration proteins showed initial burst of release. Human bone marrow stromal cells were cultured on scaffolds physisorbed with 20 mu g/mL and cultured in basal medium (DDM group) or physisorbed and cultured in osteogenic medium or cultured on non-functionalised scaffolds in osteogenic medium. The human bone marrow stromal cells proliferated less in demineralised dentine matrix group and activated ERK/1/2 after both time points. Cells on DDM group showed highest expression of IL-6 and IL-8 at 7 days and expressed higher collagen type 1 alpha 2, SPP1 and bone morphogenetic protein-2 until 21 days. Extracellular protein revealed higher collagen type 1 and bone morphogenetic protein-2 at 21 days in demineralised dentine matrix group. Cells on DDM group showed signs of mineralisation. The functionalised scaffolds were able to stimulate osteogenic differentiation of human bone marrow stromal cells.

Place, publisher, year, edition, pages
SAGE PUBLICATIONS INC , 2019. Vol. 10, article id 2041731419852703
Keywords [en]
Growth factor, mesenchymal stem cell, bone tissue engineering, drug delivery, functionalisation
National Category
Cell and Molecular Biology
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URN: urn:nbn:se:kth:diva-254028DOI: 10.1177/2041731419852703ISI: 000469789400001Scopus ID: 2-s2.0-85066462356OAI: oai:DiVA.org:kth-254028DiVA, id: diva2:1342839
Note

QC 20190814

Available from: 2019-08-14 Created: 2019-08-14 Last updated: 2019-08-14Bibliographically approved

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Fuoco, TizianaFinne Wistrand, Anna

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