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3D and Porous RGDC-Functionalized Polyester-Based Scaffolds as a Niche to Induce Osteogenic Differentiation of Human Bone Marrow Stem Cells
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Fibre- and Polymer Technology.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Fibre- and Polymer Technology.ORCID iD: 0000-0001-7135-9158
Dept Clin Dent, Arstadveien 19, N-5009 Bergen, Norway..
Dept Clin Dent, Arstadveien 19, N-5009 Bergen, Norway..
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2019 (English)In: Macromolecular Bioscience, ISSN 1616-5187, E-ISSN 1616-5195, Vol. 19, no 6, article id 1900049Article in journal (Refereed) Published
Abstract [en]

Polyester-based scaffolds covalently functionalized with arginine-glycine-aspartic acid-cysteine (RGDC) peptide sequences support the proliferation and osteogenic differentiation of stem cells. The aim is to create an optimized 3D niche to sustain human bone marrow stem cell (hBMSC) viability and osteogenic commitment, without reliance on differentiation media. Scaffolds consisting of poly(lactide-co-trimethylene carbonate), poly(LA-co-TMC), and functionalized poly(lactide) copolymers with pendant thiol groups are prepared by salt-leaching technique. The availability of functional groups on scaffold surfaces allows for an easy and straightforward method to covalently attach RGDC peptide motifs without affecting the polymerization degree. The strategy enables the chemical binding of bioactive motifs on the surfaces of 3D scaffolds and avoids conventional methods that require harsh conditions. Gene and protein levels and mineral deposition indicate the osteogenic commitment of hBMSC cultured on the RGDC functionalized surfaces. The osteogenic commitment of hBMSC is enhanced on functionalized surfaces compared with nonfunctionalized surfaces and without supplementing media with osteogenic factors. Poly(LA-co-TMC) scaffolds have potential as scaffolds for osteoblast culture and bone grafts. Furthermore, these results contribute to the development of biomimetic materials and allow a deeper comprehension of the importance of RGD peptides on stem cell transition toward osteoblastic lineage.

Place, publisher, year, edition, pages
WILEY-V C H VERLAG GMBH , 2019. Vol. 19, no 6, article id 1900049
Keywords [en]
degradable polymer, human bone marrow stem cells, poly(l-lactide-co-trimethylene carbonate), RGDC
National Category
Polymer Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-255207DOI: 10.1002/mabi.201900049ISI: 000471782900010PubMedID: 31050389Scopus ID: 2-s2.0-85065316726OAI: oai:DiVA.org:kth-255207DiVA, id: diva2:1348332
Note

QC 20190904

Available from: 2019-09-04 Created: 2019-09-04 Last updated: 2019-09-04Bibliographically approved

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Yassin, Mohammed A.Fuoco, TizianaFinne Wistrand, Anna

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