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Characterization of a natural mouse monoclonal antibody recognizing epitopes shared by oxidized low-density lipoprotein and chaperonin 60 of Aggregatibacter actinomycetemcomitans
University of Oulu, Oulu, Finland.
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2016 (English)In: Immunologic research, ISSN 0257-277X, E-ISSN 1559-0755, Vol. 64, no 3, p. 699-710Article in journal (Refereed) Published
Abstract [en]

Natural antibodies are predominantly antibodies of the IgM isotype present in the circulation of all vertebrates that have not been previously exposed to exogenous antigens. They are often directed against highly conserved epitopes and bind to ligands of varying chemical composition with low affinity. In this study we cloned and characterized a natural mouse monoclonal IgM antibody selected by binding to malondialdehyde acetaldehyde epitopes on low-density lipoprotein (LDL). Interestingly, the IgM antibody cross-reacted with Aggregatibacter actinomycetemcomitans (Aa) bacteria, a key pathogenic microbe in periodontitis reported to be associated with risk factor for atherosclerosis, thus being named as Aa_Mab. It is more intriguing that the binding molecule of Aa to Aa_Mab IgM was found to be Aa chaperonin 60 or HSP60, a member of heat-shock protein family, behaving not only as a chaperone for correct protein folding but also as a powerful virulence factor of the bacteria for inducing bone resorption and as a putative pathogenic factor in atherosclerosis. The findings will highlight the question of whether molecular mimicry between pathogen components and oxidized LDL could lead to atheroprotective immune activity, and also would be of great importance in potential application of immune response-based preventive and therapeutic strategies against atherosclerosis and periodontal disease.

Place, publisher, year, edition, pages
Humana Press, 2016. Vol. 64, no 3, p. 699-710
Keywords [en]
Aggregatibacter actinomycetemcomitans, Chaperonin 60, Malondialdehyde acetaldehyde, Natural antibody, Oxidized low-density lipoprotein
National Category
Immunology
Identifiers
URN: urn:nbn:se:kth:diva-258051DOI: 10.1007/s12026-015-8781-7ISI: 000375461800006PubMedID: 26786003Scopus ID: 2-s2.0-84954554548OAI: oai:DiVA.org:kth-258051DiVA, id: diva2:1349714
Note

QC 20190913

Available from: 2019-09-09 Created: 2019-09-09 Last updated: 2019-09-13Bibliographically approved

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Turunen, S. Pauliina
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