Catalytic Self-Screening of Cholinesterase Substrates from a Dynamic Combinatorial Thioester Library
2004 (English)In: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 43, no 28, 3716-3718 p.Article in journal (Refereed) Published
Simply the best: Dynamic combinatorial chemistry coupled to enzyme catalysis was used to identify enzyme substrates in a library constructed from a series of thioesters and thiols by transesterification. The library was directly coupled to the catalytic action of acetylcholinesterase, which selectively hydrolyzed the best substrate candidates (see schematic representation). The process allowed rapid identification of discrete substrates.
Place, publisher, year, edition, pages
2004. Vol. 43, no 28, 3716-3718 p.
combinatorial chemistry; enzyme catalysis hydrolysis; proteomics; transesterification
IdentifiersURN: urn:nbn:se:kth:diva-8265DOI: 10.1002/anie.200454165ISI: 000222793600019ScopusID: 2-s2.0-4544244707OAI: oai:DiVA.org:kth-8265DiVA: diva2:13542
QC 201008182008-04-252008-04-252011-11-01Bibliographically approved