Quartz crystal microbalance bioaffinity sensor for rapid identification of glycosyldisulfide lectin inhibitors from a dynamic combinatorial library
2006 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 22, no 1, 42-48 p.Article in journal (Refereed) Published
Carbohydrate-lectin,interactions were probed with dynamic combinatorial libraries, using the plant lectin Concanavalin A as target species. The dynamic combinatorial libraries were generated from a pool of thiol components through reversible thiol-disulfide interchange, and screened using a simple and efficient method based on a quartz crystal microbalance setup. It was found that dimers based on 1-thio- and 6-thin-mannose analogues were the most active inhibitors. Furthermore, the results clearly show that the 6-thio-mannose possess unique characteristics compared to its oxygen-containing counterpart.
Place, publisher, year, edition, pages
2006. Vol. 22, no 1, 42-48 p.
quartz crystal microbalance; dynamic combinatorial chemistry; glycochemistry; carbohydrates; lectins; molecular recognition
IdentifiersURN: urn:nbn:se:kth:diva-8269DOI: 10.1016/j.bios.2005.11.024ISI: 000241040600006ScopusID: 2-s2.0-33746607715OAI: oai:DiVA.org:kth-8269DiVA: diva2:13546
QC 201008182008-04-252008-04-252010-08-18Bibliographically approved