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Strategies for increasing the throughput of super-resolution microscopies
Ecole Polytech Fed Lausanne, Lab Expt Biophys, Inst Phys, CH-1015 Lausanne, Switzerland..ORCID iD: 0000-0003-1004-4771
KTH, School of Engineering Sciences (SCI), Applied Physics. KTH, Centres, Science for Life Laboratory, SciLifeLab.
Ecole Polytech Fed Lausanne, Lab Expt Biophys, Inst Phys, CH-1015 Lausanne, Switzerland..
Ecole Polytech Fed Lausanne, Lab Expt Biophys, Inst Phys, CH-1015 Lausanne, Switzerland..
2019 (English)In: Current opinion in chemical biology, ISSN 1367-5931, E-ISSN 1879-0402, Vol. 51, p. 84-91Article, review/survey (Refereed) Published
Abstract [en]

Fluorescence microscopy methods have been developed to circumvent the diffraction limit by exploiting nonlinearities in the interactions between light and fluorophores. Initially, these methods were up to orders of magnitude slower than standard microscopies. In recent years, a wide array of technological advances have increased the throughput of super-resolution microscopies, through parallelization, smart scanning or data processing, and sample expansion. Here, we review re cent innovations for increased throughput, some applications that have benefitted from them, and how they could be improved in the future.

Place, publisher, year, edition, pages
ELSEVIER SCI LTD , 2019. Vol. 51, p. 84-91
National Category
Biophysics
Identifiers
URN: urn:nbn:se:kth:diva-260194DOI: 10.1016/j.cbpa.2019.05.012ISI: 000483453800012PubMedID: 31212117Scopus ID: 2-s2.0-85067298035OAI: oai:DiVA.org:kth-260194DiVA, id: diva2:1355364
Note

QC 20190927

Available from: 2019-09-27 Created: 2019-09-27 Last updated: 2019-09-27Bibliographically approved

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