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Individual and combined inhibitory effects of methanol and toluene on acetyl-CoA synthetase expression level of acetoclastic methanogen, Methanosaeta concilii
Istanbul Tech Univ, Fac Civil Engn, Dept Environm Engn.ORCID iD: 0000-0002-8170-379X
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2015 (English)In: International Biodeterioration & Biodegradation, ISSN 0964-8305, E-ISSN 1879-0208Article in journal (Refereed) Published
Abstract [en]

Organic solvents found in waste streams can be inhibitory to microbial communities in wastewater treatment plants. In this study, possible inhibitory effects of commonly used organic solvents, methanol and toluene, were investigated in batch anaerobic digestion tests. Anaerobic sludges were fed with acetate repeatedly and exposed to various concentrations of toluene, methanol and mixture of them. Expression level of the key enzyme of acetoclastic methanogenesis, acetyl-CoA synthetase 1 (Acs1), was determined by reverse transcriptase real time PCR. Additionally, active populations of Methanosaeta spp. were monitored and quantified by fluorescent in situ hybridization (FISH). Transcript abundance of Acs1 was 1.31 x 10(6) mRNAs ml(-1) in the control reactors; whereas, singular methanol exposure of 0.1 M, 0.3 M, 0.5 M and 1.0 M and methanol + toluene combination of 1.0 M + 0.5 mM and 1.0 M + 1.5 mM did not cause any significant effect on the acetyl-CoA expression level. However, singular toluene-exposed serum bottle reactors were completely inhibited after 3rd exposure at all concentrations from 0.5 mM to 4.0 mM. FISH results for singular methanol and toluene additions as well as their combination in the ranges studied showed no particular effect on the relative abundance of Methanosaeta spp. cells. (C) 2015 Elsevier Ltd. All rights reserved.

Place, publisher, year, edition, pages
2015.
Keywords [en]
Acetyl-CoA synthetase; FISH; Methanol; Methanosaeta concilii; mRNA; Real-time PCR; Toluene
National Category
Other Environmental Biotechnology
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URN: urn:nbn:se:kth:diva-261391DOI: 10.1016/j.ibiod.2015.09.013ISI: 000364264300029Scopus ID: 2-s2.0-84942512377OAI: oai:DiVA.org:kth-261391DiVA, id: diva2:1357948
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QC 20191011

Available from: 2019-10-04 Created: 2019-10-04 Last updated: 2019-10-11Bibliographically approved

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