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Aldehyde-alcohol dehydrogenase forms a high-order spirosome architecture critical for its activity
Korea Adv Inst Sci & Technol, Dept Biol Sci, Daejeon 34141, South Korea..
Univ Glasgow, Inst Infect Immun & Inflammat, Glasgow G12 8QQ, Lanark, Scotland..
Korea Adv Inst Sci & Technol, Dept Biol Sci, Daejeon 34141, South Korea..
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Biomedical Engineering and Health Systems, Structural Biotechnology. Korea Adv Inst Sci & Technol, Dept Biol Sci, Daejeon 34141, South Korea..ORCID iD: 0000-0003-3862-3433
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2019 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 10, no 1, article id 4527Article in journal (Refereed) Published
Abstract [en]

Aldehyde-alcohol dehydrogenase (AdhE) is a key enzyme in bacterial fermentation, converting acetyl-CoA to ethanol, via two consecutive catalytic reactions. Here, we present a 3.5 angstrom resolution cryo-EM structure of full-length AdhE revealing a high-order spirosome architecture. The structure shows that the aldehyde dehydrogenase (ALDH) and alcohol dehydrogenase (ADH) active sites reside at the outer surface and the inner surface of the spirosome respectively, thus topologically separating these two activities. Furthermore, mutations disrupting the helical structure abrogate enzymatic activity, implying that formation of the spirosome structure is critical for AdhE activity. In addition, we show that this spirosome structure undergoes conformational change in the presence of cofactors. This work presents the atomic resolution structure of AdhE and suggests that the high-order helical structure regulates its enzymatic activity.

Place, publisher, year, edition, pages
Nature Publishing Group, 2019. Vol. 10, no 1, article id 4527
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Biochemistry and Molecular Biology
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URN: urn:nbn:se:kth:diva-262952DOI: 10.1038/s41467-019-12427-8ISI: 000489013700020PubMedID: 31586059Scopus ID: 2-s2.0-85072930837OAI: oai:DiVA.org:kth-262952DiVA, id: diva2:1374981
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QC 20191203

Available from: 2019-12-03 Created: 2019-12-03 Last updated: 2019-12-04Bibliographically approved

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Jung, TaeyangHebert, Hans

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