Electrospray Ionization from a Gap with Adjustable Width
2006 (English)In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, E-ISSN 1097-0231, Vol. 20, no 21, 3176-3182 p.Article in journal (Refereed) Published
In this paper, we present a new concept for electrospray ionization mass spectrometry, where the sample is applied in a gap which is formed between the edges of two triangular-shaped tips. The size of the spray orifice can be changed by varying the gap width. The tips were fabricated from polyethylene terephthalate film with a thickness of 36 μm. To improve the wetting of the gap and sample confinement, the edges of the tips forming the gap were hydrophilized by means of silicon dioxide deposition. Electrospray was performed with gap widths between 1 and 36 μm and flow rates down to 75 nL/min. The gap width could be adjusted in situ during the mass spectrometry experiments and nozzle clogging could be managed by simply widening the gap. Using angiotensin I as analyte, the signal-to-noise ratio increased as the gap width was decreased, and a shift towards higher charge states was observed. The detection limit for angiotensin I was in the low nM range.
Place, publisher, year, edition, pages
2006. Vol. 20, no 21, 3176-3182 p.
article, electrospray mass spectrometry, equipment, equipment design, evaluation, instrumentation, methodology, microfluidic analysis, reproducibility, sensitivity and specificity
IdentifiersURN: urn:nbn:se:kth:diva-8437DOI: 10.1002/rcm.2710ISI: 000241627000003ScopusID: 2-s2.0-33750325735OAI: oai:DiVA.org:kth-8437DiVA: diva2:13756
QC 201009132008-05-132008-05-132012-02-24Bibliographically approved