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Sorting by interfacial tension (SIFT): Label-free enzyme sorting using droplet microfluidics
Santa Clara Univ, Dept Chem & Biochem, Santa Clara, CA 95053 USA..
Santa Clara Univ, Dept Chem & Biochem, Santa Clara, CA 95053 USA..
Santa Clara Univ, Dept Chem & Biochem, Santa Clara, CA 95053 USA..
Santa Clara Univ, Dept Chem & Biochem, Santa Clara, CA 95053 USA..
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2019 (English)In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 1089, p. 108-114Article in journal (Refereed) Published
Abstract [en]

Droplet microfluidics has the ability to greatly increase the throughput of screening and sorting of enzymes by carrying reagents in picoliter droplets flowing in inert oils. It was found with the use of a specific surfactant, the interfacial tension of droplets can be very sensitive to droplet pH. This enables the sorting of droplets of different pH when confined droplets encounter a microfabricated trench. The device can be extended to sort enzymes, as a large number of enzymatic reactions lead to the production of an acidic or basic product and a concurrent change in solution pH. The progress of an enzymatic reaction is tracked from the position of a flowing train of droplets. We demonstrate the sorting of esterase isoenzymes based on their enzymatic activity. This label-free technology, that we dub droplet sorting by interfacial tension (SIFT), requires no active components and would have applications for enzyme sorting in high-throughput applications that include enzyme screening and directed evolution of enzymes.

Place, publisher, year, edition, pages
ELSEVIER , 2019. Vol. 1089, p. 108-114
Keywords [en]
Microfluidics, Droplet, Lab-on-a-chip, Sorting, Label-free, Enzyme activity
National Category
Fluid Mechanics and Acoustics
Identifiers
URN: urn:nbn:se:kth:diva-263328DOI: 10.1016/j.aca.2019.08.025ISI: 000490138000012PubMedID: 31627807Scopus ID: 2-s2.0-85071109685OAI: oai:DiVA.org:kth-263328DiVA, id: diva2:1375912
Note

QC 20191206

Available from: 2019-12-06 Created: 2019-12-06 Last updated: 2019-12-06Bibliographically approved

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Zhu, Lailai

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