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A novel tool for clinical diagnosis of allergy operating a microfluidic immunoaffinity basophil activation test technique
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science, Nano Biotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab. Department of Clinical Science and Education, Karolinska Institutet, Stockholm, Sweden.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science, Nano Biotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab. Department of Clinical Science and Education, Karolinska Institutet, Stockholm, Sweden;Sachs´ Children and Youth Hospital, Södersjukhuset, Stockholm, Sweden.
Karolinska Inst, Dept Clin Sci & Educ, Stockholm, Sweden.;Soder Sjukhuset, Sachs Children & Youth Hosp, Stockholm, Sweden..
Karolinska Univ Hosp, Dept Clin Immunol, Stockholm, Sweden..
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2019 (English)In: Clinical Immunology, ISSN 1521-6616, E-ISSN 1521-7035, Vol. 209, article id UNSP 108268Article in journal (Refereed) Published
Abstract [en]

The Basophil Activation Test (BAT) is a valuable allergy diagnostic tool but is time-consuming and requires skilled personnel and cumbersome processing, which has limited its clinical use. We therefore investigated if a microfluidic immunoaffinity BAT (miBAT) technique can be a reliable diagnostic method. Blood was collected from allergic patients and healthy controls. Basophils were challenged with negative control, positive control (anti-FccRI), and two concentrations of a relevant and non-relevant allergen. CD203c and CD63 expression was detected by fluorescent microscopy and flow cytometry. In basophils from allergic patients the CD63% was significantly higher after allergen activation as compared to the negative control (p < .0001-p = .0004). Activation with non-relevant allergen showed equivalent CD63% expression as the negative control. Further, the miBAT data were comparable to flow cytometry. Our results demonstrate the capacity of the miBAT technology to measure different degrees of basophil allergen activation by quantifying the CD63% expression on captured basophils.

Place, publisher, year, edition, pages
ACADEMIC PRESS INC ELSEVIER SCIENCE , 2019. Vol. 209, article id UNSP 108268
Keywords [en]
Basophil activation test (BAT), Microfluidic immunoaffinity basophil activation test (miBAT), IgE-antibodies, Allergen, CD63
National Category
Clinical Medicine
Identifiers
URN: urn:nbn:se:kth:diva-266423DOI: 10.1016/j.clim.2019.108268ISI: 000503321700005PubMedID: 31669191Scopus ID: 2-s2.0-85074493579OAI: oai:DiVA.org:kth-266423DiVA, id: diva2:1387444
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Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20200121

Available from: 2020-01-21 Created: 2020-01-21 Last updated: 2020-01-21Bibliographically approved

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Aljadi, ZenibKalm, FridaRussom, Aman

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