Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Phenotypic Screen with the Human Secretome Identifies FGF16 as Inducing Proliferation of iPSC-Derived Cardiac Progenitor Cells
AstraZeneca, BioPharmaceut R&D, Biosci Cardiovasc, Cardiovasc,Renal & Metab,Res & Early Dev, S-43150 Molndal, Sweden..
AstraZeneca, Mechanist Biol & Profiling, Discovery Sci, R&D, S-43150 Molndal, Sweden..
AstraZeneca, Mechanist Biol & Profiling, Discovery Sci, R&D, S-43150 Molndal, Sweden..
AstraZeneca, BioPharmaceut R&D, Biosci Cardiovasc, Cardiovasc,Renal & Metab,Res & Early Dev, S-43150 Molndal, Sweden..
Show others and affiliations
2019 (English)In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 20, no 23, article id 6037Article in journal (Refereed) Published
Abstract [en]

Paracrine factors can induce cardiac regeneration and repair post myocardial infarction by stimulating proliferation of cardiac cells and inducing the anti-fibrotic, antiapoptotic, and immunomodulatory effects of angiogenesis. Here, we screened a human secretome library, consisting of 923 growth factors, cytokines, and proteins with unknown function, in a phenotypic screen with human cardiac progenitor cells. The primary readout in the screen was proliferation measured by nuclear count. From this screen, we identified FGF1, FGF4, FGF9, FGF16, FGF18, and seven additional proteins that induce proliferation of cardiac progenitor cells. FGF9 and FGF16 belong to the same FGF subfamily, share high sequence identity, and are described to have similar receptor preferences. Interestingly, FGF16 was shown to be specific for proliferation of cardiac progenitor cells, whereas FGF9 also proliferated human cardiac fibroblasts. Biosensor analysis of receptor preferences and quantification of receptor abundances suggested that FGF16 and FGF9 bind to different FGF receptors on the cardiac progenitor cells and cardiac fibroblasts. FGF16 also proliferated naive cardiac progenitor cells isolated from mouse heart and human cardiomyocytes derived from induced pluripotent cells. Taken together, the data suggest that FGF16 could be a suitable paracrine factor to induce cardiac regeneration and repair.

Place, publisher, year, edition, pages
MDPI , 2019. Vol. 20, no 23, article id 6037
Keywords [en]
human secretome, phenotypic screening, cardiac progenitor cells, fibroblast growth factors, fibroblast growth factor 16
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-266544DOI: 10.3390/ijms20236037ISI: 000504428300219PubMedID: 31801200Scopus ID: 2-s2.0-85075880909OAI: oai:DiVA.org:kth-266544DiVA, id: diva2:1390404
Note

QC 20200131

Available from: 2020-01-31 Created: 2020-01-31 Last updated: 2020-01-31Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Tegel, HannaHober, SophiaSivertsson, ÅsaUhlén, Mathias

Search in DiVA

By author/editor
Tegel, HannaHober, SophiaSivertsson, ÅsaUhlén, Mathias
By organisation
Protein ScienceProtein Technology
In the same journal
International Journal of Molecular Sciences
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 5 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf