Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Potato Protein Nanofibrils Produced from a Starch Industry Sidestream
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Applied Physical Chemistry.ORCID iD: 0000-0002-2821-1462
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Fibre- and Polymer Technology, Polymeric Materials.
KTH, School of Engineering Sciences (SCI), Mechanics.ORCID iD: 0000-0001-5789-6299
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry.
Show others and affiliations
2020 (English)In: ACS SUSTAINABLE CHEMISTRY & ENGINEERING, ISSN 2168-0485, Vol. 8, no 2, p. 1058-1067Article in journal (Refereed) Published
Abstract [en]

Protein nanofibrils have emerged as promising building blocks in functional bio/nanomaterials as well as in food products. We here demonstrate that nanofibrils with amyloid-like properties can be produced from potato protein isolate, a major sidestream from the starch industry. Methods for solubilization of potato proteins are evaluated, and a protocol for the assembly of protein nanofibrils is presented. Characterization of the nanofibrils shows that they are rich in beta-sheet structure and display the cross-beta X-ray fiber diffraction pattern, which is a hallmark of amyloid-like fibrils. Atomic force microscopy shows that the fibrils are ca. 4-5 nm in diameter with a nanoscale morphology that displays a high degree of curvature. Using mass spectrometry we identify four peptides that constitute the core building blocks of the nanofibrils and show that they originate from two different classes of proteins. The structural characteristics of these peptides are distinct from previously studied plant protein nanofibrils and thereby reveal new knowledge about the formation of protein nanostructures from agricultural resources.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC , 2020. Vol. 8, no 2, p. 1058-1067
Keywords [en]
Potato protein, Nanomaterials, Biobased materials, Amyloid, Mass spectrometry
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-268715DOI: 10.1021/acssuschemeng.9b05865ISI: 000509432200036Scopus ID: 2-s2.0-85077661298OAI: oai:DiVA.org:kth-268715DiVA, id: diva2:1394809
Note

QC 20200220

Available from: 2020-02-20 Created: 2020-02-20 Last updated: 2020-05-11Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textScopus

Authority records BETA

Josefsson, LeilaBrett, CalvinEmmer, ÅsaLendel, Christofer

Search in DiVA

By author/editor
Josefsson, LeilaYe, XinchenBrett, CalvinMeijer, JonasOlsson, CarlSjögren, AmandaSundlöf, JosefinEmmer, ÅsaLendel, Christofer
By organisation
Applied Physical ChemistryPolymeric MaterialsMechanicsChemistry
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 25 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf