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Influence of molecular size and zeta potential in electrokinetic biosensing
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science, Protein Engineering.ORCID iD: 0000-0002-6552-8426
KTH, School of Engineering Sciences (SCI), Applied Physics, Photonics.ORCID iD: 0000-0002-5077-3218
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science.ORCID iD: 0000-0002-0695-5188
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2020 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 152, article id 112005Article in journal (Refereed) Published
Abstract [en]

Electrokinetic principles such as streaming current and streaming potential are extensively used for surface characterization. Recently, they have also been used in biosensors, resulting in enhanced sensitivity and simpler device architecture. Theoretical models regarding streaming current/potential studies of particle-covered surfaces have identified features such as the particle size, shape and surface charge to influence the electrokinetic signals and consequently, the sensitivity and effective operational regime of the biosensor. By using a set of well-characterized proteins with varying size and net surface charge, this article experimentally verifies the theoretical predictions about their influence on the sensor signal. Increasing protein size was shown to enhance the signal when their net surface charge was either opposite to that of the sensor surface, or close to zero, in agreement with the theoretical predictions. However, the effect gradually saturated as the protein size exceeded the coulomb screening length of the electrolyte. In contrast, the proteins containing the same type of charge as the surface showed little or no difference, except that the signal inverted. The magnitude of the surface charge was also shown to influence the signal. The sensitivity of the technique for protein detection varied over two orders of magnitude, depending upon the size and surface charge. Furthermore, the capacity of the electrokinetic method for direct electrical detection of various proteins, including those carrying little or no net electric charges, is demonstrated.

Place, publisher, year, edition, pages
Elsevier, 2020. Vol. 152, article id 112005
Keywords [en]
Biosensor, Electrokinetics, Improved sensitivity, Influence of molecular size and charge, Streaming current, Zeta potential
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-267788DOI: 10.1016/j.bios.2020.112005ISI: 000515210600008PubMedID: 32056733Scopus ID: 2-s2.0-85077917325OAI: oai:DiVA.org:kth-267788DiVA, id: diva2:1397763
Note

QC 20200227

Available from: 2020-02-27 Created: 2020-02-27 Last updated: 2020-05-11Bibliographically approved

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Stiller, ChristianeCavallaro, SaraEriksson Karlström, AmelieLinnros, Jan

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