Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum.
Center for Insoluble Protein Structures (inSPIN), and Interdisciplinary Nanoscience Center (iNANO) Department of Chemistry and Department of Chemistry, Aarhus C DK-8000, Denmark .
Center for Insoluble Protein Structures (inSPIN), and Interdisciplinary Nanoscience Center (iNANO) Department of Chemistry and Department of Chemistry, Aarhus C DK-8000, Denmark.
Center for Insoluble Protein Structures (inSPIN), and Interdisciplinary Nanoscience Center (iNANO) Department of Chemistry and Department of Chemistry, Aarhus C DK-8000, Denmark.
Institute of Physics, University of Tartu, Estonia.
Show others and affiliations
2016 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 7, article id 12454Article in journal (Refereed) Published
Abstract [en]

Photosynthetic antenna systems enable organisms harvesting light and transfer the energy to the photosynthetic reaction centre, where the conversion to chemical energy takes place. One of the most complex antenna systems, the chlorosome, found in the photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum contains a baseplate, which is a scaffolding super-structure, formed by the protein CsmA and bacteriochlorophyll a. Here we present the first high-resolution structure of the CsmA baseplate using intact fully functional, light-harvesting organelles from Cba. tepidum, following a hybrid approach combining five complementary methods: solid-state NMR spectroscopy, cryo-electron microscopy, isotropic and anisotropic circular dichroism and linear dichroism. The structure calculation was facilitated through development of new software, GASyCS for efficient geometry optimization of highly symmetric oligomeric structures. We show that the baseplate is composed of rods of repeated dimers of the strongly amphipathic CsmA with pigments sandwiched within the dimer at the hydrophobic side of the helix.

Place, publisher, year, edition, pages
2016. Vol. 7, article id 12454
National Category
Natural Sciences
Research subject
Biological Physics
Identifiers
URN: urn:nbn:se:kth:diva-233011DOI: 10.1038/ncomms12454PubMedID: 27534696Scopus ID: 2-s2.0-84983283811OAI: oai:DiVA.org:kth-233011DiVA, id: diva2:1410959
Available from: 2020-03-02 Created: 2020-03-02 Last updated: 2020-05-11

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Jegerschöld, Caroline
By organisation
School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH)
In the same journal
Nature Communications
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf