Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Poly(ethylene glycol) Self-Assembled Monolayer Island Growth
KTH, School of Engineering Sciences (SCI), Applied Physics, Nanostructure Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics, Nanostructure Physics.ORCID iD: 0000-0001-8534-6577
2005 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 21, 2981-2987 p.Article in journal (Refereed) Published
Abstract [en]

Here, we report a study of the morphology and growth dynamics of a self-assembled monolayer (SAM) of the amide containing poly(ethylene glycol) (PEG) thiol (CH3O(CH2CH2O)(17)NHCO(CH2)(2)SH) on atomically flat Au(111) surfaces. SAM growth from a 20 mu M ethanolic solution reveals island growth through three distinct steps: island nucleation, island growth, and coalescence. The coalescence-step, filling voids in the SAM, is by far slowest. The fine structure study reveals dendritic island formation, an observation which can be explained by attractive intermolecular interactions and surface diffusion-limited aggregation. We have also observed a change in the island height, which peaks during the island growth phase. This height change can be associated with a molecular conformational transition.

Place, publisher, year, edition, pages
2005. Vol. 21, 2981-2987 p.
Keyword [en]
sum-frequency spectroscopy, size distribution, surface modification, protein adsorption, hydrogen-bonds, in-situ, gold, alkanethiols, microscopy, mechanisms
National Category
Physical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-8845DOI: 10.1021/la0471792ISI: 000228042400052Scopus ID: 2-s2.0-16244376824OAI: oai:DiVA.org:kth-8845DiVA: diva2:14309
Note
QC 20101008Available from: 2005-12-02 Created: 2005-12-02 Last updated: 2017-12-14Bibliographically approved
In thesis
1. Nanometer Scale Protein Templates for Bionanotechnology Applications
Open this publication in new window or tab >>Nanometer Scale Protein Templates for Bionanotechnology Applications
2005 (English)Doctoral thesis, comprehensive summary (Other scientific)
Abstract [en]

Nanofabrication techniques were used to manufacture nanometer scale protein templates. The fabrication approach employs electron beam lithography (EBL) patterning on poly(ethylene glycol) (PEG) thiol (CH3O(CH2CH2O)17NHCO(CH2)2SH) self-assembled monolayers (SAM) on Au. The PEG SAM prevented protein surface adhesion and binding sites for protein were created in the SAM by EBL. Subsequent to EBL, the patterns in the PEG SAM were backfilled with 40-nm NeutrAvidin-coated fluorescent spheres (FluoSpheres). The spontaneous and directed immobilization of the spheres from a solution to the patterns resulted in high resolution protein patterns. The FluoSpheres could be arranged in any arbitrary pattern with ultimately only one or a few FluoSpheres at each binding site.

Growth dynamics and SAM morphology of PEG on Au were studied by atomic force microscopy (AFM). PEG SAMs on three types of Au with different microstructure were examined: thermally evaporated granular Au and two types of Au films produced by hydrogen flame annealing of granular Au, Au(111) and "terraced" Au (crystal orientation unknown). The different Au surfaces' substructure affected the morphology and mechanical properties of the PEG SAM. On Au(111), AFM imaging revealed monolayer formation through three distinct steps: island nucleation, island growth, and coalescence. The fine-structure of the SAM revealed dendritic island formation - an observation which can be explained by attractive intermolecular interactions and diffusion-limited aggregation. Island growth was not observed on the "terraced" Au.

AFM studies of EBL patterned PEG SAMs on Au(111) revealed two different patterning mechanisms. At low doses, the pattern formation occurs by SAM ablation in a self-developing process where the feature depth is directly dose dependent. At higher doses electron beam induced deposition of material, so-called contamination writing, is seen in the ablated areas of the SAM. The balance between these two mechanisms is shown to depend on the geometry of the pattern.

In addition to PEG SAMs, fibronectin monolayers on SiO2 surfaces were patterned by EBL. The areas exposed with EBL lose their functionality and do not bind anti-fibronectin. With this approach we constructed fibronectin templates and used them for cell studies demonstrating pattern dependent cell geometries and cell adhesion.

Place, publisher, year, edition, pages
Stockholm: KTH, 2005. xv, 108 p.
Series
Trita-FYS, ISSN 0280-316X ; 2005:65
Keyword
nanotechnology, bionanotechnology, nanobiotechnology, electron beam lithography, EBL, poly(ethylene glycol), PEG, self-assembled monolayer, SAM, self-immobilization, protein pattern
National Category
Physical Sciences
Identifiers
urn:nbn:se:kth:diva-530 (URN)91-7178-178-1 (ISBN)
Public defence
2005-12-09, FA32, AlbaNova, Roslagstullsbacken 21, Stockholm, 10:15
Opponent
Supervisors
Note
QC 20101008Available from: 2005-12-02 Created: 2005-12-02 Last updated: 2012-03-21Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopushttp://pubs.acs.org/cgi-bin/abstract.cgi/langd5/2005/21/i07/abs/la0471792.html

Authority records BETA

Haviland, David B.

Search in DiVA

By author/editor
Rundqvist, JonasHoh, Jan H.Haviland, David B.
By organisation
Nanostructure Physics
In the same journal
Langmuir
Physical Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 89 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf