Directed Immobilization of Protein-Coated Nanospheres toNanometer-Scale Patterns Fabricated by Electron Beam Lithographyof Poly(ethylene glycol) Self-Assembled Monolayers
2006 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 22, no 11, 5100-5107 p.Article in journal (Refereed) Published
Controlling the spatial organization of biomolecules on solid supports with high resolution is important for a widerange of scientific and technological problems. Here we report a study of electron beam lithography (EBL) patterningof a self-assembled monolayer (SAM) of the amide-containing poly(ethylene glycol) (PEG) thiol CH3O(CH2CH2O)17-NHCO(CH2)2SH on Au and demonstrate the patterning of biomolecular features with dimensions approaching 40 nm.The electron dose dependence of feature size and pattern resolution is studied in detail by atomic force microscopy(AFM), which reveals two distinct patterning mechanisms. At low doses, the pattern formation occurs bySAMablationin a self-developing process where the feature size is directly dose-dependent. At higher doses, electron beam-induceddeposition of material, so-called contamination writing, is seen in the ablated areas of the SAM. The balance betweenthese two mechanisms is shown to depend on the geometry of the pattern. The patterned SAMs were backfilled withfluorescent 40-nm spheres coated with NeutrAvidin. These protein-coated spheres adhered to exposed areas in theSAM with high selectivity. This direct writing approach for patterning bioactive surfaces is a fast and efficient wayto produce patterns with a resolution approaching that of single proteins.
Place, publisher, year, edition, pages
2006. Vol. 22, no 11, 5100-5107 p.
scanning force microscopy, chemical nanolithography, deposited tips, surfaces, silicon, adsorption, biosensor, resists
IdentifiersURN: urn:nbn:se:kth:diva-8847DOI: 10.1021/la052306vISI: 000237593000035ScopusID: 2-s2.0-33745736728OAI: oai:DiVA.org:kth-8847DiVA: diva2:14311
Uppdaterad från "In press" till published: 20101008.
Tidigare titel: Directed Protein Adhesion to Nanometer Scale Patterns Fabricated by Electron Beam Lithography on Poly(ethylene glycol) Self-Assembled Monolayers
QC 201010082005-12-022005-12-022010-10-08Bibliographically approved