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Immobilization of Enamel Matrix Derivate Protein onto Polypeptide Multilayers: Comparative in Situ Measurements using Ellipsometry, Quartz Crystal Microbalance with Dissipation, and Dual Polarization Interferometry
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface Chemistry.
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Surface Chemistry.
2006 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 22, no 26, 11065-11071 p.Article in journal (Refereed) Published
Abstract [en]

The buildup of biodegradable poly(L-glutamic acid) (PGA) and poly(L-lysine) (PLL) multilayers on silica and titanium surfaces and the immobilization of enamel matrix derivate (EMD) protein was followed by utilizing in situ ellipsometry, quartz crystal microbalance with dissipation, and dual-polarization interferometry (DPI). The use of the relatively new DPI technique validated earlier published ellipsometry measurements of the PLL-PGA polypeptide films. The hydrophobic aggregating EMD protein was successfully immobilized both on top of and within the multilayer structures at pH 5.0. DPI measurements further indicated that the immobilization of EMD is influenced by the flow pattern during adsorption. The formed polypeptide-EMD multilayer films are of interest since it is known that EMD is able to trigger cell response and induce biomineralization. The multilayer films thus have potential to be useful as bioactive and biodegradable coatings for future dental implants.

Place, publisher, year, edition, pages
2006. Vol. 22, no 26, 11065-11071 p.
Keyword [en]
Dual-polarization interferometry, Enamel matrix derivate proteins, Polypeptide multilayers, Quartz crystal microbalance
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-8922DOI: 10.1021/la0607712ISI: 000242771200035Scopus ID: 2-s2.0-33846380249OAI: oai:DiVA.org:kth-8922DiVA: diva2:14408
Note
QC 20100917. Uppdaterad från Manuskript till Artikel (20100917)Available from: 2005-12-08 Created: 2005-12-08 Last updated: 2017-12-14Bibliographically approved
In thesis
1. Multilayer Structures for Biomaterial Applications: Biomacromolecule-based Coatings
Open this publication in new window or tab >>Multilayer Structures for Biomaterial Applications: Biomacromolecule-based Coatings
2005 (English)Doctoral thesis, comprehensive summary (Other scientific)
Abstract [en]

The cellular response to a biomaterial, such as a dental implant, is mainly governed by the surface properties, and can thus be altered by the introduction of a surface coating. In this thesis the buildup of a biomacromolecule-based coating formed by layerby-layer (LbL) deposition of the charged polypeptides poly(L-lysine) (PLL) and poly(L-glutamic acid) (PGA) has been studied. In an attempt to make these coatings bioactive and useful for bone-anchored implants, an amelogenin protein mixture (EMD), has been immobilized in these thin polyelectrolyte multilayer (PEM) films. Multilayers were also built by LbL deposition of the natural biomacromolecules collagen (Col) and hyaluronic acid (HA). Multilayer films of these two extra-cellular biomacromolecules should be of interest for use as a scaffold for tissue engineering.

The buildup of the multilayer films has been followed in situ, using ellipsometry, quartz crystal microbalance with dissipation (QCM-D), and dual polarization interferometry (DPI). The studied PLL/PGA multilayers were found to be highly hydrated, and to exhibit a two-regime buildup behavior, with an initial “slow-growing” regime, and a second “fast-growing” regime with a linear growth in film thickness and more than linear growth in mass. A net diffusion of polypeptides into the film during the buildup led to an increase in density of the films for each layer adsorbed. A change in density was also observed in the Col/HA film, where HA penetrated and diffused into the porous fibrous Col network.

The formed PLL/PGA films were further found to be rather stable during drying, and post-buildup changes in temperature and pH, not losing any mass as long as the temperature was not raised too rapidly. The film thickness responded to changes in the ambient media and collapsed reversibly when dried. A swelling/de-swelling behavior of the film was also observed for changes in the temperature and pH.

The EMD protein adsorbed to silica surfaces as nanospheres, and could by itself form multilayers. The adsorption of EMD onto PLL/PGA multilayer films increased at lower pH (5.0), and EMD could be immobilized in several layers by alternate deposition of EMD and PGA.

Place, publisher, year, edition, pages
Stockholm: KTH, 2005. xvii, 67 p.
Series
Trita-YTK, ISSN 1650-0490 ; 0505
Keyword
multilayer, layer-by-layer deposition, physical chemistry, surface chemistry, adsorption, ellipsometry, QCM-D, DPI, protein adsorption, polypeptides, biomaterials, biosurfaces, amelogenin, solid/liquid interface
National Category
Physical Chemistry
Identifiers
urn:nbn:se:kth:diva-551 (URN)91-7178-216-8 (ISBN)
Public defence
2005-12-16, F3, KTH, Lindstedtsvägen 26, Stockholm, 13:00
Opponent
Supervisors
Note
QC 20101019Available from: 2005-12-08 Created: 2005-12-08 Last updated: 2010-10-19Bibliographically approved

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