Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Calcium phosphate precipitation in blood serum model solutions
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Inorganic Chemistry.
2005 (English)Licentiate thesis, comprehensive summary (Other scientific)
Place, publisher, year, edition, pages
Stockholm: KTH , 2005. , xiv, 45 p.
Series
Trita-OOK, ISSN 0348-825X ; 1082
National Category
Inorganic Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-576ISBN: 91-7178-217-6 (print)OAI: oai:DiVA.org:kth-576DiVA: diva2:14482
Presentation
2005-12-15, sal K2, KTH, Teknikringen 28, Stockholm, 14:00
Opponent
Supervisors
Note
QC 20101220Available from: 2005-12-28 Created: 2005-12-28 Last updated: 2010-12-20Bibliographically approved
List of papers
1. A theoretical investigation of the supersaturation of basic calcium phosphate in serum of dialysis patients
Open this publication in new window or tab >>A theoretical investigation of the supersaturation of basic calcium phosphate in serum of dialysis patients
2006 (English)In: Journal of Applied Biomaterials and Biomechanics, ISSN 1722-6899, Vol. 4, no 2, 80-86 p.Article, review/survey (Refereed) Published
Abstract [en]

Extraosseous calcification in hemodialysis (HD) patients consists mainly of biological apatite, idealized as hydroxyapatite. Other suggested calcium phosphates are octacalcium phosphate (OCP) and brushite, both known to be hydroxyapatite precursors. Whatever the mechanisms of mineral deposition are, these mechanisms are always required to produce a supersaturated state, and that state can be calculated from the solubility product (SP) of the relevant mineral. Supersaturation in relation to serum ionized calcium [Ca2+] and total inorganic serum phosphate (Pi) under normal and hyperphosphatemic conditions has been calculated. While supersaturation with respect to hydroxyapatite and OCP always exists, and supersaturation with respect to Ca5(PO4)3(HCO3)·4H2O is just above the limit, supersaturation with respect to brushite solely occurs under hyperphosphatemic conditions. In order to avoid supersaturation with respect to brushite the maximum serum phosphate level allowed is 1.9 mmol/L (5.8 mg/dl) and the calcium-phosphate product (Ca x— P) 4.5 (mmol/L)2 (56 (mg/dl))2.

Keyword
Biomineralization, Brushite, Calcification, Calcium phosphate, Hyperphosphatemia, calcium, hydroxyapatite, phosphate, calcium blood level, hemodialysis patient, human, phosphate blood level, review
National Category
Inorganic Chemistry
Identifiers
urn:nbn:se:kth:diva-27710 (URN)20799206 (PubMedID)2-s2.0-33749648142 (Scopus ID)
Note

QC 20101220

Available from: 2010-12-20 Created: 2010-12-20 Last updated: 2014-04-07Bibliographically approved
2. Formation of carbonated apatite particles from a supersaturated inorganic blood serum model
Open this publication in new window or tab >>Formation of carbonated apatite particles from a supersaturated inorganic blood serum model
Show others...
2009 (English)In: Journal of materials science. Materials in medicine, ISSN 0957-4530, E-ISSN 1573-4838, Vol. 20, no 8, 1677-1687 p.Article in journal (Refereed) Published
Abstract [en]

Pathological calcification is common among for instance dialysis patients, and this causes an increase in mortality risk. An elevated serum phosphate concentration among those patients strongly correlates to this increase. In this work investigations of the conditions, composition, crystallinity and morphology of in vitro calcification are performed and related to results from in vivo studies. The study was performed under conditions mimicking physiological ones, i.e. a pH around 7.40, a temperature of 37A degrees C, an ionic strength of 150 mM and ion concentrations close to those in human serum including the effects of elevated phosphate concentrations. The course of precipitation involves an initial precipitate that subsequently re-dissolves to give another precipitate, in accordance with the well-known Ostwald ripening theory. The final bulk precipitate consists of a macroscopically amorphous carbonated apatite. The amorphous apatite is formed from assemblies of spherical particles in the mu m range, in turn composed of nano-crystalline needles of about 10 x 100 nm. Even the initially formed precipitate, as well as a small amount of precipitate that occurs on the liquid surface, consist of a carbonated calcium phosphate. The in vitro observed carbonated apatite bears strong resemblance to in vivo cardiovascular calcification known from literature.

National Category
Inorganic Chemistry
Identifiers
urn:nbn:se:kth:diva-18615 (URN)10.1007/s10856-009-3735-z (DOI)000268103300012 ()2-s2.0-68149124109 (Scopus ID)
Note
QC 20100525Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2011-01-19Bibliographically approved
3. The observation of nano-crystalline calcium phosphate precipitate in a simple supersaturated inorganic blood serum model - composition and morphology
Open this publication in new window or tab >>The observation of nano-crystalline calcium phosphate precipitate in a simple supersaturated inorganic blood serum model - composition and morphology
2009 (English)In: Journal of Applied Biomaterials and Biomechanics, ISSN 1722-6899, Vol. 7, no 1, 13-22 p.Article in journal (Refereed) Published
Abstract [en]

Aim. Calcium phosphate deposition in blood vessels is correlated to increased mortality risk. In this study, the formation of solid calcium phosphate in an in vitro solution mimicking the inorganic part of blood serum was studied. Methods. The precipitates formed were analyzed using several experimental techniques, including infrared spectroscopy, Raman spectroscopy, X-ray energy dispersive spectroscopy, chemical analysis of combustion gases, thermogravimetric analysis, as well as transmission electron microscopy and scanning electron microscopy. Results. The results indicate a microscopically amorphous but nano-crystalline material with an overall apatite structure. A plausible stoichiometry was determined to Ca-5(PO4)(3)(HCO3)center dot 4H(2)O with an estimated solubility constant of 6.10(-39) (mol/L)(9). Bicarbonate in the water solution was shown to be essential for the precipitation, giving implications for in vitro studies. Conclusions. The calcium phosphate formed in this study shows many similarities to pathological calcium phosphates regarding composition, morphology and crystallinity.

Keyword
Apatite, Carbonated apatite, Mineralization, Nano-crystalline
National Category
Inorganic Chemistry
Identifiers
urn:nbn:se:kth:diva-18697 (URN)000269103400002 ()20740434 (PubMedID)2-s2.0-68149130919 (Scopus ID)
Note
QC 20100525Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2010-12-20Bibliographically approved
4. In vitro formation of nanocrystalline carbonate apatite - A structural and morphological analogue of atherosclerotic plaques
Open this publication in new window or tab >>In vitro formation of nanocrystalline carbonate apatite - A structural and morphological analogue of atherosclerotic plaques
2007 (English)In: European Journal of Inorganic Chemistry, ISSN 1434-1948, E-ISSN 1099-0682, no 26, 4123-4127 p.Article in journal (Refereed) Published
Abstract [en]

The in vitro formation of carbonate apatite in solutions with ion concentrations comparable to those in human serum was studied. The composition and morphology of the resulting apatite precipitate displayed a hierarchical assembly of elongated plate-shaped nanocrystals of carbonate apatite analogous to previously characterized bioapatites formed in The main conclusion is that so-called bioapatites may form in vitro and that precipitation inhibitors most likely are essential for the prevention of spontaneous calcification at normal human serum ion concentrations.

Keyword
carbonate apatite, crystallization, calcification, nanocrystalline, calcium, calcification, dialysis, serum, hydroxyapatite, morphogenesis, phosphate, arteries, protein, growth, mice
National Category
Inorganic Chemistry
Identifiers
urn:nbn:se:kth:diva-16978 (URN)10.1002/ejic.200700654 (DOI)000249767700003 ()2-s2.0-34748836558 (Scopus ID)
Note
QC 20100525. Tidigare titel: "Spontaneous in vitro Calcification in Physiological Salt and Buffer Solution with Normal Human Serum Ionized Calcium and Inorganic Phosphate Concentrations".Available from: 2010-08-05 Created: 2010-08-05 Last updated: 2010-12-20Bibliographically approved

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Sandin, Karin
By organisation
Inorganic Chemistry
Inorganic Chemistry

Search outside of DiVA

GoogleGoogle Scholar

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 522 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf