Five folding box boards made on the same paperboard machine have been analyzed. The paperboards were from the same product series but had different grammage (235, 255, 270, 315, 340 g/m2) and different bending stiffness. The paperboards are normally used to make packages, and since the bending stiffness and grammage varies the packages performance will be different. Finite element simulations can be used to predict these differences. However, the stiffness and strength properties then need to be known. For efficient determination of the three-dimensional properties in MD, CD and ZD, it is proposed that the whole paperboard should be characterized with the following tests: in-plane tension, ZD tension, shear strength profiles and two-point bending. The stiffness and strength properties have with the proposed setups been determined at different relative humidity (20, 50, 70 and 90 % RH), and the mechanical properties have been evaluated as function of moisture ratio. The results showed a linear relation between mechanical properties and moisture ratio for each paperboard. The data was then normalized with data for the standard climate (50 % RH) and investigated as a function of moisture ratio. The results indicated that the normalized mechanical properties for all paperboards coincided along one single line and could therefore be expressed as a linear function of moisture ratio and two constants. Consequently, the study indicates that it is possible to obtain the mechanical properties of a paperboard, by knowing the structural properties for the preferred level of RH and the mechanical property for the standard climate (50 % RH and 23 °C).