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Fluorescent Probes for STED Optical Nanoscopy
Daegu Gyeongbuk Inst Sci & Technol, Dept New Biol, Daegu 42988, South Korea..
KTH, School of Engineering Sciences (SCI), Applied Physics, Quantum and Biophotonics.ORCID iD: 0000-0003-3200-0374
Daegu Gyeongbuk Inst Sci & Technol, Dept New Biol, Daegu 42988, South Korea.;Daegu Gyeongbuk Inst Sci & Technol, New Biol Res Ctr, Daegu 42988, South Korea..
2022 (English)In: Nanomaterials, E-ISSN 2079-4991, Vol. 12, no 1, article id 21Article in journal (Refereed) Published
Abstract [en]

Progress in developing fluorescent probes, such as fluorescent proteins, organic dyes, and fluorescent nanoparticles, is inseparable from the advancement in optical fluorescence microscopy. Super-resolution microscopy, or optical nanoscopy, overcame the far-field optical resolution limit, known as Abbe's diffraction limit, by taking advantage of the photophysical properties of fluorescent probes. Therefore, fluorescent probes for super-resolution microscopy should meet the new requirements in the probes' photophysical and photochemical properties. STED optical nanoscopy achieves super-resolution by depleting excited fluorophores at the periphery of an excitation laser beam using a depletion beam with a hollow core. An ideal fluorescent probe for STED nanoscopy must meet specific photophysical and photochemical properties, including high photostability, depletability at the depletion wavelength, low adverse excitability, and biocompatibility. This review introduces the requirements of fluorescent probes for STED nanoscopy and discusses the recent progress in the development of fluorescent probes, such as fluorescent proteins, organic dyes, and fluorescent nanoparticles, for the STED nanoscopy. The strengths and the limitations of the fluorescent probes are analyzed in detail.

Place, publisher, year, edition, pages
MDPI AG , 2022. Vol. 12, no 1, article id 21
Keywords [en]
super-resolution microscopy, STED, fluorescent probe, fluorescent protein, organic dye, fluorescent nanoparticle
National Category
Atom and Molecular Physics and Optics Biophysics Other Physics Topics
Identifiers
URN: urn:nbn:se:kth:diva-307558DOI: 10.3390/nano12010021ISI: 000742980200001PubMedID: 35009972Scopus ID: 2-s2.0-85121431041OAI: oai:DiVA.org:kth-307558DiVA, id: diva2:1633482
Note

QC 20220131

Available from: 2022-01-31 Created: 2022-01-31 Last updated: 2022-06-25Bibliographically approved

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Widengren, Jerker

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