Despite widespread awareness that enormous consumption of plastics is not sustainable, the global production and use of plastics continue to grow. This generates vast amounts of plastic waste and micmplastics, ending up e. g., in the marine environment. There are serious challenges in detecting and measuring microplastics, especially in highly diluted natural samples. Here, a new alternative microplastic detection method based on plasmon-enhanced fluorescence (PEF) was developed and tested using fluorescence microscopy. In particular, gold nanopillar-based substrates, displaying (i) high electromagnetic field enhancement, and (ii) surface super-hydrophobicity and high adhesion properties, were utilized to enhance the fluorescence emission signal from microplastics in water samples. The fluorescence microscopy imaging revealed remarkable fluorescence enhancement by the PEF substrates on the microplastic particles and fibers with different sizes of both conventional, low-density polyethylene, and biodegradable poly (butylene adipate-co-terephthalate). The limit of detection and quantification by this method was estimated to be as low as 0.35 and 1.2 femtograms, respectively. The observed fluorescence enhancement of the gold nanopillar substrates for the microplastics was ca. 70 times greater than the case of having the microplastics on a glass substrate. Additionally, 3D FEM simulations were performed to further investigate the system's electromagnetic field distribution near the nanostructures. This new method makes undyed microplastics visible in fluorescence microscopy, even particles and fibres too small to be imaged with conventional light microscopy. This can be a great tool for micmplastic research, helping us to detect, study, understand microplastic dynamics in water based systems.